PMID:23066145

Citation	Kou, X, Watkins, CB and Gan, SS (2012) Arabidopsis AtNAP regulates fruit senescence. J. Exp. Bot. 63:6139-47
Abstract	Arabidopsis has been used as a model system to study many aspects of plant growth and development. However, fruit senescence in Arabidopsis has been less investigated and the underlying molecular and hormonal (especially ethylene) regulatory mechanisms are not well understood. It is reported here that the Arabidopsis silique has characteristics of a climacteric fruit, and that AtNAP, a NAC family transcription factor gene whose expression is increased with the progression of silique senescence, plays an important role in its senescence. Silique senescence was delayed for 4-5 d in the atnap knockout mutant plants. The ethylene climacteric was delayed for 2 d in the atnap silique and the associated respiratory climacteric was suppressed. Exogenous ethylene stimulated respiration in the wild type, but not in the atnap mutant. The decoupling of the ethylene and respiratory climacterics in the atnap mutant suggests that AtNAP is required for ethylene stimulation of respiration. qPCR analyses revealed that the expression patterns of genes involved in ethylene biosynthesis, perception, and signalling, ACS2, ETR1, CTR1, EIN2, EIN3, and ERF1, were also altered in the atnap mutant. The effects of exogenous ABA, SA, 6-BA, and NAA on ethylene production and respiration in siliques of the wild type and atnap mutant were also investigated. A model involving ABA-AtNAP-controlled stomatal opening in regulating ethylene-stimulated respiration in fruit senescence is presented.
Links	PubMed PMC3481206 Online version:10.1093/jxb/ers266
Keywords	Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Respiration; Chlorophyll/metabolism; Ethylenes/analysis; Ethylenes/pharmacology; Fruit/physiology; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Knockout Techniques; Models, Biological; Mutation; Phenotype; Plant Leaves/drug effects; Plant Leaves/genetics; Plant Leaves/growth & development; Plant Leaves/physiology; Plant Stomata/drug effects; Plant Stomata/genetics; Plant Stomata/growth & development; Plant Stomata/physiology; Plants, Genetically Modified; RNA, Plant/genetics; Signal Transduction; Time Factors; Transcription Factors/genetics; Transcription Factors/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:NAC29		GO:0009835: fruit ripening	ECO:0000315:		P		Figures 1a-c demonstrate delayed silique senescence in atnap knockout plants when compared to age-matched wildtype.	complete CACAO 8207
ARATH:NAC29	involved_in	GO:0009835: fruit ripening	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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