PMID:23029546

Citation	Murphy, SV, Lim, R, Heraud, P, Cholewa, M, Le Gros, M, de Jonge, MD, Howard, DL, Paterson, D, McDonald, C, Atala, A, Jenkin, G and Wallace, EM (2012) Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator. PLoS ONE 7:e46533
Abstract	Cystic fibrosis, an autosomal recessive disorder caused by a mutation in a gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR), remains a leading cause of childhood respiratory morbidity and mortality. The respiratory consequences of cystic fibrosis include the generation of thick, tenacious mucus that impairs lung clearance, predisposing the individual to repeated and persistent infections, progressive lung damage and shortened lifespan. Currently there is no cure for cystic fibrosis. With this in mind, we investigated the ability of human amnion epithelial cells (hAECs) to express functional CFTR. We found that hAECs formed 3-dimensional structures and expressed the CFTR gene and protein after culture in Small Airway Growth Medium (SAGM). We also observed a polarized CFTR distribution on the membrane of hAECs cultured in SAGM, similar to that observed in polarized airway cells in vivo. Further, hAECs induced to express CFTR possessed functional iodide/chloride (I(-/)Cl(-)) ion channels that were inhibited by the CFTR-inhibitor CFTR-172, indicating the presence of functional CFTR ion channels. These data suggest that hAECs may be a promising source for the development of a cellular therapy for cystic fibrosis.
Links	PubMed PMC3460882 Online version:10.1371/journal.pone.0046533
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:CFTR		GO:0030324: lung development	ECO:0000269:		P		Figure 2 shows that functional CFTR was grown on epithelial cells. CFTR if mutated in the genes can cause susceptibility to a large amount of lung diseases.	complete CACAO 6461

References

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