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PMID:23027764
| Citation |
Luan, W, Fesseler, J, Chechik, M, Buttner, CR, Antson, AA and Smits, C (2012) Recombinant portal protein from Staphylococcus epidermidis bacteriophage CNPH82 is a 13-subunit oligomer. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68:1267-70 |
|---|---|
| Abstract |
The portal protein cn3 of bacteriophage CNPH82 is predicted to serve as a gateway for translocation of viral genome into preformed pro-capsid, like portal proteins from other double-stranded DNA tailed bacteriophages. The host of bacteriophage CNPH82 is the opportunistic human pathogenic bacterium Staphylococcus epidermidis, a major cause of nosocomial infections. The portal protein of this phage has been cloned, overexpressed and purified. Size-exclusion chromatography-multi-angle laser light scattering analysis has indicated that the portal protein contains ∼13 subunits. Crystals of the portal protein, diffracting to 4.2 Å, have been obtained. These crystals belong to the space group C222(1) with the unit-cell parameters of a = 252.4, b = 367.0, c = 175.5 Å. The self-rotation function revealed the presence of a single 13-subunit oligomer in the asymmetric unit. |
| Links |
PubMed PMC3490468 Online version:10.1107/S1744309112037645 |
| Keywords |
Protein Subunits/chemistry; Recombinant Proteins/chemistry; Staphylococcus Phages/chemistry; Staphylococcus epidermidis/virology; Viral Proteins/chemistry |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0046798: viral portal complex |
ECO:0000314: |
C |
This protein forms the portal complex for the bacteriophage CNPH82. It has a 13-fold symmetry that was calculated using the stereo-graphic projections of Figure 3. |
complete | ||||
|
part_of |
GO:0046798: viral portal complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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