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PMID:23022439

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Citation

Couturier, J, Vignols, F, Jacquot, JP and Rouhier, N' (2012) Glutathione- and glutaredoxin-dependent reduction of methionine sulfoxide reductase A. FEBS Lett. '

Abstract

A natural fusion occurring between two tandemly repeated glutaredoxin (Grx) modules and a methionine sulfoxide reductase A (MsrA) has been detected in Gracilaria gracilis. Using an in vivo yeast complementation assay and in vitro activity measurements, we demonstrated that this fusion enzyme was able to reduce methionine sulfoxide into methionine using glutathione as a reductant. Consistently, a poplar cytosolic MsrA can be regenerated in vitro by glutaredoxins with an efficiency comparable to that of thioredoxins, but using a different mechanism. We hypothesize that the glutathione/glutaredoxin system could constitute an evolutionary conserved alternative regeneration system for MsrA.

Links

PubMed Online version:10.1016/j.febslet.2012.09.020

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

GRAGA:Q9XHG1

GO:0004362: glutathione-disulfide reductase activity

ECO:0000314:

F

Figure 2

complete
CACAO 5439

GRAGA:Q9XHG1

enables

GO:0004362: glutathione-disulfide reductase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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