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PMID:23010750
| Citation |
Yang, Z, Zhang, Y, Shen, T, Xie, Y, Mao, Y and Ji, C (2013) Cloning, expression and biochemical characterization of a novel, moderately thermostable GDSL family esterase from Geobacillus thermodenitrificans T2. J. Biosci. Bioeng. 115:133-7 |
|---|---|
| Abstract |
A thermostable GDSL family esterase-encoding gene, EstL5, was directly obtained from the genomic DNA of Geobacillus thermodenitrificans T2. Recombinant hexahistidine-tagged EstL5 was overexpressed, purified, and its biochemical properties were partially characterized. EstL5 was observed to be active within the temperature range of 0-80°C, having maximal activity at 60°C. Unlike most other thermostable enzymes, EstL5 displayed 24% of its highest activity at 0°C. EstL5 exhibited a high level of activity within a pH range of 6.0-11.0, showing the highest activity at pH 8.0. EstL5 also retained 100% of its activity after a 12-h incubation at 55°C. Furthermore, this enzyme was observed to be strongly inhibited by 10% (w/v) SDS and 0.1 mM PMSF. |
| Links |
PubMed Online version:10.1016/j.jbiosc.2012.08.016 |
| Keywords |
Amino Acid Motifs; Amino Acid Sequence; Cloning, Molecular; Enzyme Stability; Esterases/antagonists & inhibitors; Esterases/chemistry; Esterases/genetics; Esterases/isolation & purification; Esterases/metabolism; Geobacillus/enzymology; Geobacillus/genetics; Hydrogen-Ion Concentration; Indicators and Reagents/pharmacology; Models, Molecular; Molecular Sequence Data; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Temperature |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016788: hydrolase activity, acting on ester bonds |
ECO:0000314: |
F |
Table 2 |
complete | ||||
|
enables |
GO:0016788: hydrolase activity, acting on ester bonds |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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