GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:22977246
| Citation |
'Kulczyk, AW, Akabayov, B, Lee, SJ, Bostina, M, Berkowitz, SA and Richardson, CC (2012) An Interaction between DNA Polymerase and Helicase is Essential for the High Processivity of the Bacteriophage T7 Replisome. J. Biol. Chem. ' |
|---|---|
| Abstract |
Synthesis of the leading DNA strand requires the coordinated activity of DNA polymerase and DNA helicase while synthesis of the lagging-strand involves interactions of these proteins with DNA primase. We present the first structural model of a bacteriophage T7 DNA helicase/DNA polymerase complex using a combination of SAXS, single-molecule and biochemical methods. We propose that the protein-protein interface stabilizing the leading-strand synthesis involves two distinct interactions: a stable binding of the helicase to the palm domain of the polymerase and an electrostatic binding of the C-terminal tail of the helicase to a basic patch on the polymerase. DNA primase facilitates binding of DNA helicase to ssDNA and contributes to formation of the DNA helicase/DNA polymerase complex by stabilizing DNA helicase. |
| Links |
PubMed Online version:10.1074/jbc.M112.410647 |
| Keywords |
|
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
See also
References
See Help:References for how to manage references in GONUTS.
