PMID:22911780

Citation	Molesini, B, Pandolfini, T, Pii, Y, Korte, A and Spena, A (2012) Arabidopsis thaliana AUCSIA-1 regulates auxin biology and physically interacts with a kinesin-related protein. PLoS ONE 7:e41327
Abstract	Aucsia is a green plant gene family encoding 44-54 amino acids long miniproteins. The sequenced genomes of most land plants contain two Aucsia genes. RNA interference of both tomato (Solanum lycopersicum) Aucsia genes (SlAucsia-1 and SlAucsia-2) altered auxin sensitivity, auxin transport and distribution; it caused parthenocarpic development of the fruit and other auxin-related morphological changes. Here we present data showing that the Aucsia-1 gene of Arabidopsis thaliana alters, by itself, root auxin biology and that the AtAUCSIA-1 miniprotein physically interacts with a kinesin-related protein. The AtAucsia-1 gene is ubiquitously expressed, although its expression is higher in roots and inflorescences in comparison to stems and leaves. Two allelic mutants for AtAucsia-1 gene did not display visible root morphological alterations; however both basipetal and acropetal indole-3-acetic acid (IAA) root transport was reduced as compared with wild-type plants. The transcript steady state levels of the auxin efflux transporters ATP BINDING CASSETTE subfamily B (ABCB) ABCB1, ABCB4 and ABCB19 were reduced in ataucsia-1 plants. In ataucsia-1 mutant, lateral root growth showed an altered response to i) exogenous auxin, ii) an inhibitor of polar auxin transport and iii) ethylene. Overexpression of AtAucsia-1 inhibited primary root growth. In vitro and in vivo protein-protein interaction experiments showed that AtAUCSIA-1 interacts with a 185 amino acids long fragment belonging to a 2712 amino acids long protein of unknown function (At4g31570). Bioinformatics analysis indicates that the AtAUCSIA-1 interacting protein (AtAUCSIA-1IP) clusters with a group of CENP-E kinesin-related proteins. Gene ontology predictions for the two proteins are consistent with the hypothesis that the AtAUCSIA-1/AtAUCSIA-1IP complex is involved in the regulation of the cytoskeleton dynamics underlying auxin biology.
Links	PubMed PMC3401106 Online version:10.1371/journal.pone.0041327
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:Q9MAC4		GO:0009926: auxin polar transport	ECO:0000315:		P		Figure 3A shows reduced polar auxin transport in aucsia-1 mutants.	complete CACAO 5421
ARATH:Q9MAC4		GO:0009733: response to auxin stimulus	ECO:0000315:		P		Figure 4 shows that aucsia-1 mutants have an altered response to auxins compared to the wild-type.	complete CACAO 5422
ARATH:Q9MAC4	involved_in	GO:0009926: auxin polar transport	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:Q9MAC4	involved_in	GO:0009733: response to auxin	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

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