PMID:22859673

Citation	Kou, Y, Chang, Y, Li, X, Xiao, J and Wang, S (2012) The rice RAD51C gene is required for the meiosis of both female and male gametocytes and the DNA repair of somatic cells. J. Exp. Bot. 63:5323-35
Abstract	The RecA/RAD51 family of rice (Oryza sativa) consists of at least 13 members. However, the functions of most of these members are unknown. Here the functional characterization of one member of this family, RAD51C, is reported. Knockout (KO) of RAD51C resulted in both female and male sterility in rice. Transferring RAD51C to the RAD51C-KO line restored fertility. Cytological analyses showed that the sterility of RAD51C-KO plants was associated with abnormal early meiotic processes in both megasporocytes and pollen mother cells (PMCs). PMCs had an absence of normal pachytene chromosomes and had abnormal chromosome fragments. The RAD51C-KO line showed no obvious difference from wild-type plants in mitosis in the anther wall cells, which was consistent with the observation that the RAD51C-KO line did not have obviously abnormal morphology during vegetative development. However, the RAD51C-KO line was sensitive to different DNA-damaging agents. These results suggest that RAD51C is essential for reproductive development by regulating meiosis as well as for DNA damage repair in somatic cells.
Links	PubMed PMC3431001 Online version:10.1093/jxb/ers190
Keywords	Agrobacterium/genetics; Chromosomes, Plant/drug effects; Chromosomes, Plant/metabolism; Chromosomes, Plant/radiation effects; DNA Fragmentation/drug effects; DNA Fragmentation/radiation effects; DNA Repair/drug effects; DNA Repair/radiation effects; DNA, Bacterial/genetics; Gene Knockout Techniques; Genes, Plant; Genetic Complementation Test; Germ Cells, Plant/drug effects; Germ Cells, Plant/growth & development; Germ Cells, Plant/radiation effects; Meiosis/drug effects; Meiosis/radiation effects; Mitosis/drug effects; Mitosis/radiation effects; Molecular Sequence Data; Mutagens/pharmacology; Oryza sativa/cytology; Oryza sativa/drug effects; Oryza sativa/genetics; Oryza sativa/growth & development; Phylogeny; Plant Infertility; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Genetically Modified; Pollen/drug effects; Pollen/growth & development; Pollen/radiation effects; Rad51 Recombinase/genetics; Rad51 Recombinase/metabolism; Sequence Analysis, DNA
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ORYSA:J9PFC8	involved_in	GO:0007141: male meiosis I	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ORYSA:J9PFC8		GO:0007141: male meiosis I	ECO:0000315:		P		Figure 6.	complete CACAO 8131
ORYSA:J9PFC8		GO:0007143: female meiosis	ECO:0000315:		P		Figure 3.	complete CACAO 8132
ORYSA:J9PFC8		GO:0006281: DNA repair	ECO:0000315:		P		Figure 8.	complete CACAO 8133
ORYSA:J9PFC8	involved_in	GO:0006281: DNA repair	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ORYSA:J9PFC8	involved_in	GO:0007143: female meiotic nuclear division	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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