PMID:22835533

Citation	Zhang, M, Pan, J, Kong, X, Zhou, Y, Liu, Y, Sun, L and Li, D (2012) ZmMKK3, a novel maize group B mitogen-activated protein kinase kinase gene, mediates osmotic stress and ABA signal responses. J. Plant Physiol. 169:1501-10
Abstract	Mitogen-activated protein kinase (MAPK) cascades are important intracellular signaling modules and function as a convergent point for crosstalk during abiotic stress signaling. In this article, we isolated a novel group B MAPKK gene, ZmMKK3, from Zea mays. ZmMKK3 protein might be localized in both the cytoplasm and the nucleus. RNA blot analysis indicated that the ZmMKK3 transcription was up-regulated by abscisic acid (ABA), hydrogen peroxide (H(2)O(2)) and PEG, and that H(2)O(2) mediated PEG-induced expression of ZmMKK3. Constitutive expression of ZmMKK3 in Nicotiana tabacum reduced H(2)O(2) accumulation under osmotic stress by affecting antioxidant defense systems and alleviated reactive oxygen species-mediated injury under oxidative stress. Transgenic tobacco exhibited attenuated ABA sensitivity by means of an increased germination rate and main root growth. Taken together, these results indicate that ZmMKK3 is a positive regulator of osmotic tolerance and ABA signaling in plants.
Links	PubMed Online version:10.1016/j.jplph.2012.06.008
Keywords	Abscisic Acid/metabolism; Gene Expression Regulation, Plant; Genes, Plant; Hydrogen Peroxide/metabolism; Mitogen-Activated Protein Kinases/genetics; Mitogen-Activated Protein Kinases/metabolism; Osmotic Pressure/physiology; Plants, Genetically Modified/metabolism; Polyethylene Glycols/metabolism; Signal Transduction/genetics; Stress, Physiological; Tobacco/enzymology; Tobacco/growth & development; Zea mays/enzymology; Zea mays/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MAIZE:J9PPQ1	involved_in	GO:0047484: regulation of response to osmotic stress	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MAIZE:J9PPQ1		GO:0004708: MAP kinase kinase activity	ECO:0000266:	UniProtKB:O49975 UniProtKB:Q40542	F		Figure 1b	complete CACAO 7989
MAIZE:J9PPQ1		GO:0047484: regulation of response to osmotic stress	ECO:0000314:		P		Figures 5a and 5b	complete CACAO 7990

References

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