GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:22787217
| Citation |
Kurochkina, LP, Semenyuk, PI, Orlov, VN, Robben, J, Sykilinda, NN and Mesyanzhinov, VV (2012) Expression and functional characterization of the first bacteriophage-encoded chaperonin. J. Virol. 86:10103-11 |
|---|---|
| Abstract |
Chaperonins promote protein folding in vivo and are ubiquitously found in bacteria, archaea, and eukaryotes. The first viral chaperonin GroEL ortholog, gene product 146 (gp146), whose gene was earlier identified in the genome of bacteriophage EL, has been shown to be synthesized during phage propagation in Pseudomonas aeruginosa cells. The recombinant gp146 has been expressed in Escherichia coli and characterized by different physicochemical methods for the first time. Using serum against the recombinant protein, gp146's native substrate, the phage endolysin gp188, has been immunoprecipitated from the lysate of EL-infected bacteria and identified by mass spectrometry. In vitro experiments have shown that gp146 has a protective effect against endolysin thermal inactivation and aggregation, providing evidence of its chaperonin function. The phage chaperonin has been found to have the architecture and some properties similar to those of GroEL but not to require cochaperonin for its functional activity. |
| Links |
PubMed PMC3446618 Online version:10.1128/JVI.00940-12 |
| Keywords |
Base Sequence; Chaperonins/chemistry; Chaperonins/genetics; Chaperonins/metabolism; DNA, Viral/genetics; Microscopy, Electron, Transmission; Multiprotein Complexes; Protein Denaturation; Protein Multimerization; Pseudomonas Phages/genetics; Pseudomonas Phages/metabolism; Pseudomonas aeruginosa/virology; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Thermodynamics; Viral Proteins/chemistry; Viral Proteins/genetics; Viral Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016465: chaperonin ATPase complex |
ECO:0000314: |
C |
Figure 7 shows ADP and ATP binding to recombinant gp146 Figure 8 B compares gp146 and gp188* with and without 3mM of ATP and shows that gp188* in the presence of gp146 and ATP is not affected. |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
