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PMID:22771964
| Citation |
Seo, JK, Lee, MJ, Go, HJ, Park, TH and Park, NG (2012) Purification and characterization of YFGAP, a GAPDH-related novel antimicrobial peptide, from the skin of yellowfin tuna, Thunnus albacares. Fish Shellfish Immunol. 33:743-52 |
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| Abstract |
A 3.4 kDa of antimicrobial peptide was purified from an acidified skin extract of the yellowfin tuna, Thunnus albacares, by preparative acid-urea-polyacrylamide gel electrophoresis and C(18) reversed-phase HPLC. A comparison of the N-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high homology with the N-terminus of glyceraldehyde-3-phosphate dehydrogenase (GAPDH); thus, this peptide was designated as the yellowfin tuna GAPDH-related antimicrobial peptide (YFGAP). YFGAP showed potent antimicrobial activity against Gram-positive bacteria, such as Bacillus subtilis, Micrococcus luteus, and Streptococcus iniae (minimal effective concentrations [MECs], 1.2-17.0 μg/mL), and Gram-negative bacteria, such as Aeromonas hydrophila, Escherichia coli D31, and Vibrio parahaemolyticus (MECs, 3.1-12.0 μg/mL) without significant hemolytic activity. According to the secondary structural prediction and the homology modeling, this peptide forms an amphipathic structure and consists of three secondary structural motifs including one α-helix and two parallel β-strands. This peptide did not show membrane permeabilization ability and its activity was bacteriostatic rather than bactericidal. This is the first report of the isolation of an antimicrobial peptide from a tuna species and the first description of the antimicrobial function of the N-terminus of GAPDH of an animal species. |
| Links |
PubMed Online version:10.1016/j.fsi.2012.06.023 |
| Keywords |
Amino Acid Sequence; Animals; Antimicrobial Cationic Peptides/chemistry; Antimicrobial Cationic Peptides/metabolism; Bacillus subtilis/immunology; Escherichia coli/immunology; Glyceraldehyde-3-Phosphate Dehydrogenases/chemistry; Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism; Protein Structure, Secondary; Sequence Homology, Amino Acid; Skin/metabolism; Tuna/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0002775: antimicrobial peptide production |
ECO:0000255: |
|
P |
Figure 3 showing ESI-MS results with properties common to antimicrobial peptides |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
- ↑ Narvaez, E et al. (2010) An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus. Fish Shellfish Immunol. 28 235-9 PubMed GONUTS page
