PMID:22750300

Citation	Lee, OR, Kim, YJ, Balusamy, SR, Khorolragchaa, A, Sathiyaraj, G, Kim, MK and Yang, DC (2012) Expression of the ginseng PgPR10-1 in Arabidopsis confers resistance against fungal and bacterial infection. Gene 506:85-92
Abstract	Korean ginseng (Panax ginseng C. A. Meyer) consists of nine cultivars from three Jakyung, Chungkyung, and Hwangsook lines. Among three previously identified PR-10 homologs from ginseng (PgPR10-1, PgPR10-2, and PgPR10-3), we found that the exact same sequence of PgPR10-2 exist in all tested nine cultivars. But a deletion and SNP was found in American ginseng (Panax quinquefolius). PR-10 proteins are known to be small and cytosolic, and showed similar three-dimensional structure. Here we show that the heterologous overexpression of PgPR10-1 in Arabidopsis showed enhanced resistance against Pseudomonas syringe, Fusarium oxysporum, and Botrytis cinerea and in-frame tagging with fluorescent protein showed its cytoplasm and nucleus localization. Protein-protein interaction of PgPR10-2 with PgPR10-1, PgPR10-2 and PgPR10-3 suggests that the PgPR10 proteins might form multimeric complexes in different cellular compartments to function in development and in defense-related mechanism. Differential response of PgPR10-1 and PgPR10-2 against different sets of biotic stresses in ginseng plant supports this notion.
Links	PubMed Online version:10.1016/j.gene.2012.06.039
Keywords	Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/microbiology; Base Sequence; Botrytis/pathogenicity; Conserved Sequence; DNA, Plant/genetics; Fusarium/pathogenicity; Genetic Vectors; Molecular Sequence Data; Panax/genetics; Panax/metabolism; Panax/microbiology; Phylogeny; Plant Diseases/genetics; Plant Diseases/microbiology; Plant Proteins/chemistry; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Genetically Modified; Pseudomonas syringae/pathogenicity; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Stress, Physiological
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PANGI:E9M219	involved_in	GO:0050829: defense response to Gram-negative bacterium	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
PANGI:E9M219		GO:0050829: defense response to Gram-negative bacterium	ECO:0000315:		P		Figure 5A-D	complete CACAO 8325
PANGI:E9M219		GO:0050832: defense response to fungus	ECO:0000315:		P		Figure 5E-H	complete CACAO 8326
PANGI:E9M219	involved_in	GO:0050832: defense response to fungus	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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