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PMID:22611190
| Citation |
Veesler, D, Spinelli, S, Mahony, J, Lichière, J, Blangy, S, Bricogne, G, Legrand, P, Ortiz-Lombardia, M, Campanacci, V, van Sinderen, D and Cambillau, C (2012) Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism. Proc. Natl. Acad. Sci. U.S.A. 109:8954-8 |
|---|---|
| Abstract |
Phages of the Caudovirales order possess a tail that recognizes the host and ensures genome delivery upon infection. The X-ray structure of the approximately 1.8 MDa host adsorption device (baseplate) from the lactococcal phage TP901-1 shows that the receptor-binding proteins are pointing in the direction of the host, suggesting that this organelle is in a conformation ready for host adhesion. This result is in marked contrast with the lactococcal phage p2 situation, whose baseplate is known to undergo huge conformational changes in the presence of Ca(2+) to reach its active state. In vivo infection experiments confirmed these structural observations by demonstrating that Ca(2+) ions are required for host adhesion among p2-like phages (936-species) but have no influence on TP901-1-like phages (P335-species). These data suggest that these two families rely on diverse adhesion strategies which may lead to different signaling for genome release. |
| Links |
PubMed PMC3384155 Online version:10.1073/pnas.1200966109 |
| Keywords |
Bacteriophage P2/genetics; Calcium/metabolism; Caudovirales/genetics; Crystallography; Lactococcus lactis/virology; Models, Molecular; Viral Tail Proteins/chemistry; Viral Tail Proteins/genetics; Viral Tail Proteins/metabolism; Virus Attachment |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016339: calcium-dependent cell-cell adhesion |
ECO:0000314: |
P |
See Figure 4. |
complete | ||||
| GO:0098025 : virus tail, baseplate |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
|
part_of |
GO:0098025: virus tail, baseplate |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0098025: virus tail, baseplate |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
|
part_of |
GO:0098025: virus tail, baseplate |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0098025: virus tail, baseplate |
ECO:0000314: |
C |
Fig. 1 |
complete | ||||
|
part_of |
GO:0098025: virus tail, baseplate |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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