PMID:22611190

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Citation

Veesler, D, Spinelli, S, Mahony, J, Lichière, J, Blangy, S, Bricogne, G, Legrand, P, Ortiz-Lombardia, M, Campanacci, V, van Sinderen, D and Cambillau, C (2012) Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism. Proc. Natl. Acad. Sci. U.S.A. 109:8954-8

Abstract

Phages of the Caudovirales order possess a tail that recognizes the host and ensures genome delivery upon infection. The X-ray structure of the approximately 1.8 MDa host adsorption device (baseplate) from the lactococcal phage TP901-1 shows that the receptor-binding proteins are pointing in the direction of the host, suggesting that this organelle is in a conformation ready for host adhesion. This result is in marked contrast with the lactococcal phage p2 situation, whose baseplate is known to undergo huge conformational changes in the presence of Ca(2+) to reach its active state. In vivo infection experiments confirmed these structural observations by demonstrating that Ca(2+) ions are required for host adhesion among p2-like phages (936-species) but have no influence on TP901-1-like phages (P335-species). These data suggest that these two families rely on diverse adhesion strategies which may lead to different signaling for genome release.

Links

PubMed PMC3384155 Online version:10.1073/pnas.1200966109

Keywords

Bacteriophage P2/genetics; Calcium/metabolism; Caudovirales/genetics; Crystallography; Lactococcus lactis/virology; Models, Molecular; Viral Tail Proteins/chemistry; Viral Tail Proteins/genetics; Viral Tail Proteins/metabolism; Virus Attachment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

9CAUD:Q38183

GO:0016339: calcium-dependent cell-cell adhesion

ECO:0000314:

P

See Figure 4.

complete
CACAO 5202

9CAUD:Q9G096

GO:0098025 : virus tail, baseplate

ECO:0000314:

C

Figure 1.

complete
CACAO 5435

9CAUD:Q9G096

part_of

GO:0098025: virus tail, baseplate

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

9CAUD:Q9AZ58

GO:0098025: virus tail, baseplate

ECO:0000314:

C

Figure 1.

complete
CACAO 5440

9CAUD:Q9AZ58

part_of

GO:0098025: virus tail, baseplate

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

9CAUD:Q9AZ56

GO:0098025: virus tail, baseplate

ECO:0000314:

C

Fig. 1

complete
CACAO 5441

9CAUD:Q9AZ56

part_of

GO:0098025: virus tail, baseplate

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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