PMID:22500103

Citation	Bañuelos, C, García-Rivera, G, López-Reyes, I, Mendoza, L, González-Robles, A, Herranz, S, Vincent, O and Orozco, E (2012) EhADH112 is a Bro1 domain-containing protein involved in the Entamoeba histolytica multivesicular bodies pathway. J. Biomed. Biotechnol. 2012:657942
Abstract	EhADH112 is an Entamoeba histolytica Bro1 domain-containing protein, structurally related to mammalian ALIX and yeast BRO1, both involved in the Endosomal Sorting Complexes Required for Transport (ESCRT)-mediated multivesicular bodies (MVB) biogenesis. Here, we investigated an alternative role for EhADH112 in the MVB protein trafficking pathway by overexpressing 166 amino acids of its N-terminal Bro1 domain in trophozoites. Trophozoites displayed diminished phagocytosis rates and accumulated exogenous Bro1 at cytoplasmic vesicles which aggregated into aberrant complexes at late stages of phagocytosis, probably preventing EhADH112 function. Additionally, the existence of a putative E. histolytica ESCRT-III subunit (EhVps32) presumably interacting with EhADH112, led us to perform pull-down experiments with GST-EhVps32 and [(35)S]-labeled EhADH112 or EhADH112 derivatives, confirming EhVps32 binding to EhADH112 through its Bro1 domain. Our overall results define EhADH112 as a novel member of ESCRT-accessory proteins transiently present at cellular surface and endosomal compartments, probably contributing to MVB formation during phagocytosis.
Links	PubMed PMC3303925 Online version:10.1155/2012/657942
Keywords	Adhesins, Bacterial/chemistry; Adhesins, Bacterial/metabolism; Calcium-Binding Proteins/chemistry; Cell Cycle Proteins/chemistry; Cell Membrane/metabolism; Endosomal Sorting Complexes Required for Transport/chemistry; Endosomal Sorting Complexes Required for Transport/genetics; Endosomal Sorting Complexes Required for Transport/metabolism; Entamoeba histolytica/metabolism; Histocytochemistry; Humans; Models, Molecular; Phagocytosis/physiology; Protozoan Proteins/chemistry; Protozoan Proteins/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Trophozoites/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ENTHI:Q9U7F6		GO:0032509: endosome transport via multivesicular body sorting pathway	ECO:0000314:		P		Figure 6 shows antibody tagged EhADH112 localizing along the MVB membrane after the introduction of RBC	complete CACAO 4831
ENTHI:Q9U7F6	involved_in	GO:0032509: endosome transport via multivesicular body sorting pathway	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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