PMID:22479468

Citation	Yan, X, Zhang, L, Chen, B, Xiong, Z, Chen, C, Wang, L, Yu, J, Lu, C and Wei, W (2012) Functional identification and characterization of the Brassica napus transcription factor gene BnAP2, the ortholog of Arabidopsis thaliana APETALA2. PLoS ONE 7:e33890
Abstract	BnAP2, an APETALA2 (AP2)-like gene, has been isolated from Brassica napus cultivar Zhongshuang 9. The cDNA of BnAP2, with 1, 299 bp in length, encoded a transcription factor comprising of 432 amino acid residues. Results from complementary experiment indicated that BnAP2 was completely capable of restoring the phenotype of Arabidopsis ap2-11 mutant. Together with the sequence and expression data, the complementation data suggested that BnAP2 encodes the ortholog of AtAP2. To address the transcriptional activation of BnAP2, we performed transactivation assays in yeast. Fusion protein of BnAP2 with GAL4 DNA binding domain strongly activated transcription in yeast, and the transactivating activity of BnAP2 was localized to the N-terminal 100 amino acids. To further study the function of BnAP2 involved in the phenotype of B. napus, we used a transgenic approach that involved targeted RNA interference (RNAi) repression induced by ihp-RNA. Floral various phenotype defectives and reduced female fertility were observed in B. napus BnAP2-RNAi lines. Loss of the function of BnAP2 gene also resulted in delayed sepal abscission and senescence with the ethylene-independent pathway. In the strong BnAP2-RNAi lines, seeds showed defects in shape, structure and development and larger size. Strong BnAP2-RNAi and wild-type seeds initially did not display a significant difference in morphology at 10 DAF, but the development of BnAP2-RNAi seeds was slower than that of wild type at 20 DAF, and further at 30 DAF, wild-type seeds were essentially at their final size, whereas BnAP2-RNAi seeds stopped growing and developing and gradually withered.
Links	PubMed PMC3314020 Online version:10.1371/journal.pone.0033890
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BRANA:E7EAV8		GO:0080187: floral organ senescence	ECO:0000315:		P		Figure 9 shows AP2-RNAi plants have a blocked progression of senescence compared with that of the wild type	complete CACAO 5393
BRANA:E7EAV8		GO:0048316: seed development	ECO:0000315:		P		Figure 11 shows that AP2-RNAi plants have attenuated seed development	complete CACAO 5391
BRANA:E7EAV8		GO:0048461: flower structural organization	ECO:0000315:		P		Figure 7 shows AP2-RNAi lines have severe alterations in flower identity and development	complete CACAO 5392
BRANA:E7EAV8		GO:0010227: floral organ abscission	ECO:0000315:		P		Figure 9	complete CACAO 8213
BRANA:E7EAV8	involved_in	GO:0010227: floral organ abscission	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
BRANA:E7EAV8	involved_in	GO:0080187: floral organ senescence	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
BRANA:E7EAV8	involved_in	GO:0048461: flower structural organization	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
BRANA:E7EAV8	involved_in	GO:0048316: seed development	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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