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PMID:22470547
| Citation |
Carneiro, P, Duarte, M and Videira, A (2012) Characterization of apoptosis-related oxidoreductases from Neurospora crassa. PLoS ONE 7:e34270 |
|---|---|
| Abstract |
The genome from Neurospora crassa presented three open reading frames homologous to the genes coding for human AIF and AMID proteins, which are flavoproteins with oxidoreductase activities implicated in caspase-independent apoptosis. To investigate the role of these proteins, namely within the mitochondrial respiratory chain, we studied their cellular localization and characterized the respective null mutant strains. Efficiency of the respiratory chain was analyzed by oxygen consumption studies and supramolecular organization of the OXPHOS system was assessed through BN-PAGE analysis in the respective null mutant strains. The results demonstrate that, unlike in mammalian systems, disruption of AIF in Neurospora does not affect either complex I assembly or function. Furthermore, the mitochondrial respiratory chain complexes of the mutant strains display a similar supramolecular organization to that observed in the wild type strain. Further characterization revealed that N. crassa AIF appears localized to both the mitochondria and the cytoplasm, whereas AMID was found exclusively in the cytoplasm. AMID2 was detected in both mitochondria and cytoplasm of the amid mutant strain, but was barely discernible in wild type extracts, suggesting overlapping functions for the two proteins. |
| Links |
PubMed PMC3314617 Online version:10.1371/journal.pone.0034270 |
| Keywords |
Amino Acid Sequence; Apoptosis; Apoptosis Inducing Factor/analysis; Apoptosis Inducing Factor/metabolism; Apoptosis Regulatory Proteins/analysis; Apoptosis Regulatory Proteins/metabolism; Cytoplasm/metabolism; Electron Transport Complex I/genetics; Electron Transport Complex I/metabolism; Fungal Proteins/analysis; Fungal Proteins/metabolism; Mitochondria/metabolism; Molecular Sequence Data; Mutation; Neurospora crassa/enzymology; Neurospora crassa/genetics; Neurospora crassa/metabolism; Oxidoreductases/analysis; Oxidoreductases/metabolism; Sequence Alignment |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005737: cytoplasm |
ECO:0000314: |
C |
Figure 2A shows by western blot that AIF appears in the PMS (cytoplasm) fraction. |
complete | ||||
| GO:0005739: mitochondrion |
ECO:0000314: |
C |
Figure 2A shows that AIF "was also consistently detected in purified mitochondria" |
complete | ||||
| GO:0005737: cytoplasm |
ECO:0000314: |
C |
Figure 2A shows by western blot that AMID appears in the cytoplasmic (PMS) fraction. |
complete | ||||
|
part_of |
GO:0005737: cytoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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