PMID:22465360

Citation	Garcia-Orozco, KD, Sanchez-Paz, A, Aispuro-Hernandez, E, Gomez-Jimenez, S, Lopez-Zavala, A, Araujo-Bernal, S and Muhlia-Almazan, A (2012) Gene expression and protein levels of thioredoxin in the gills from the whiteleg shrimp (Litopenaeus vannamei) infected with two different viruses: the WSSV or IHHNV. Fish Shellfish Immunol. 32:1141-7
Abstract	The thioredoxin (TRX) system in crustaceans has demonstrated to act as a cell antioxidant being part of the immune response by dealing with the increased production of reactive oxygen species during bacterial or viral infection. Since the number of marine viruses has increased in the last years significantly affecting aquaculture practices of penaeids, and due to the adverse impact on wild and cultured shrimp populations, it is important to elucidate the dynamics of the shrimp response to viral infections. The role of Litopenaeus vannamei thioredoxin (LvTRX) was compared at both, mRNA and protein levels, in response to two viruses, the white spot syndrome virus (WSSV) and the infectious hypodermal and hematopoietic necrosis virus (IHHNV). The results confirmed changes in the TRX gene expression levels of WSSV-infected shrimp, but also demonstrated a more conspicuous response of TRX to WSSV than to IHHNV. While both the dimeric and monomeric forms of LvTRX were detected by Western blot analysis during the WSSV infection, the dimer on its reduced form was only detected through the IHHNV infectious process. These findings indicate that WSSV or IHHNV infected shrimp may induce a differential response of the LvTRX protein.
Links	PubMed Online version:10.1016/j.fsi.2012.03.020
Keywords	Animals; Densovirinae/physiology; Gene Expression Profiling; Gene Expression Regulation; Gills/immunology; Gills/virology; Penaeidae/immunology; Penaeidae/virology; Thioredoxins/genetics; Thioredoxins/immunology; Time Factors; White spot syndrome virus 1/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PENVA:B1PWB9		GO:0055114: oxidation-reduction process	ECO:0000366:		P		Figure 5. Fig. 5. LvTrx in gills extracts western blot. Reduced dimer (RD) and monomer (RM), and oxidized forms of TRX dimer (OD) and monomer (OM) in the protein extract of gill from the white shrimp L. vannamei. (A) Analysis from shrimp challenged with WSSV. Lane M, molecular weight markers; lane rLvTRX1, recombinant protein LvTRX as positive control; lanes 3e8, gill extracts from challenged shrimp at 0, 6, 12, 24, 36 and 48 h post-injection, respectively. (B) Analysis from shrimp challenged with IHHNV. Lane M, molecular weight markers; lane rLvTRX1, recombinant protein LvTRX as positive control; lanes 3e6, gill extracts from challenged shrimp at 0, 7, 14 and 21 days, respectively. Fig. 6. Semiquantitative values of protein bands intensity (pixels). TRX dimeric and monomeric forms during viral infection. A) WSSV infected shrimp. B) IHHNV infected shrimp. Organism: Litopenaues vannamei Uniprot protein: Thioredoxin	complete

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