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PMID:22457634
| Citation |
Rodrigues, CD and Harry, EJ (2012) The Min system and nucleoid occlusion are not required for identifying the division site in Bacillus subtilis but ensure its efficient utilization. PLoS Genet. 8:e1002561 |
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| Abstract |
Precise temporal and spatial control of cell division is essential for progeny survival. The current general view is that precise positioning of the division site at midcell in rod-shaped bacteria is a result of the combined action of the Min system and nucleoid (chromosome) occlusion. Both systems prevent assembly of the cytokinetic Z ring at inappropriate places in the cell, restricting Z rings to the correct site at midcell. Here we show that in the bacterium Bacillus subtilis Z rings are positioned precisely at midcell in the complete absence of both these systems, revealing the existence of a mechanism independent of Min and nucleoid occlusion that identifies midcell in this organism. We further show that Z ring assembly at midcell is delayed in the absence of Min and Noc proteins, while at the same time FtsZ accumulates at other potential division sites. This suggests that a major role for Min and Noc is to ensure efficient utilization of the midcell division site by preventing Z ring assembly at potential division sites, including the cell poles. Our data lead us to propose a model in which spatial regulation of division in B. subtilis involves identification of the division site at midcell that requires Min and nucleoid occlusion to ensure efficient Z ring assembly there and only there, at the right time in the cell cycle. |
| Links |
PubMed PMC3310732 Online version:10.1371/journal.pgen.1002561 |
| Keywords |
Bacillus subtilis/genetics; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Cycle Checkpoints/genetics; Cell Division/genetics; Cellular Structures/cytology; Cellular Structures/metabolism; Chromosomes, Bacterial; Cytokinesis/genetics; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000917: barrier septum assembly |
ECO:0000270: |
P |
Figure 2 shows FtsZ overproduction reduces the delay in Z ring assembly at midcell in the noc minCD double mutant. |
complete | ||||
See also
References
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