PMID:22451392

Citation	Kobayashi, T, Uchimura, K, Koide, O, Deguchi, S and Horikoshi, K (2012) Genetic and biochemical characterization of the Pseudoalteromonas tetraodonis alkaline κ-carrageenase. Biosci. Biotechnol. Biochem. 76:506-11
Abstract	An alkaline κ-carrageenase, Cgk-K142, was found in the culture broth of a deep-sea bacterium, Pseudoalteromonas tetraodonis JAM-K142. A gene for the enzyme was cloned and expressed. Purified recombinant Cgk-K142 (rCgk-K142) showed an optimal pH of about 8.8 in glycine-NaOH buffer at 30 °C and of about 8.0 in MOPS buffer at 50 °C. The optimal temperature for the enzyme was 55 °C at pH 8.0. rCgk-K142 was unstable, but λ- and ι-carrageenans, non-degradative substrate homologs, extensively enhanced its stability. The nucleotide sequence of the gene for Cgk-K142 comprised 1,194 bp, and the deduced amino acid sequence (397 amino acids) showed a high level of similarity to the κ-carrageenase of P. carrageenovora, with 94% identity. Another gene for a κ-carrageenase-like protein was found downstream of the gene for Cgk-K142. The nucleotide sequence of that gene consisted of 966 bp (321 amino acids), and it showed the highest similarity, at 64% identity, to protein CgkB of P. carrageenovora, which has been reported as an incomplete 57-amino acid sequence.
Links	PubMed
Keywords	Amino Acid Sequence; Base Sequence; Cloning, Molecular; Glycoside Hydrolases/chemistry; Glycoside Hydrolases/genetics; Glycoside Hydrolases/isolation & purification; Glycoside Hydrolases/metabolism; Hydrogen-Ion Concentration; Molecular Sequence Data; Pseudoalteromonas/enzymology; Pseudoalteromonas/genetics; RNA, Bacterial/genetics; RNA, Ribosomal, 16S/genetics; Sequence Analysis, RNA; Substrate Specificity; Temperature
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9GAMM:E7FKF4		GO:0033918: kappa-carrageenase activity	ECO:0000314:		F		Figure 5.	complete CACAO 8411
9GAMM:E7FKF4	enables	GO:0033918: kappa-carrageenase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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