PMID:22415789

Citation	Ramani, G, Meera, B, Vanitha, C, Rao, M and Gunasekaran, P (2012) Production, purification, and characterization of a β-glucosidase of Penicillium funiculosum NCL1. Appl. Biochem. Biotechnol. 167:959-72
Abstract	Penicillium funiculosum NCL1, a filamentous fungus, produced significantly higher levels of β-glucosidase. The effect of initial pH, incubation temperature, and different carbon sources on extracellular β-glucosidase production was studied in submerged fermentation. At 30 °C with initial pH 5.0, enzyme production was increased by 48-fold upon induction with paper mill waste, as compared to commercial cellulose powder. In zymogram analysis, four isoforms of β-glucosidases were observed with wheat bran whereas a minimum of one isoform was observed with other carbon sources. A major β-glucosidase (Bgl3A) with the apparent molecular weight of ~120 kDa, induced by paper mill waste, was purified 19-fold to homogeneity, with a specific activity of 1,796 U/mg. Bgl3A was a monomeric glycoprotein with 29% of neutral carbohydrate content. It showed optimum activity at pH 4.0 and 5.0, optimum temperature at 60 °C, and exhibited a half-life of 1 h at 60 °C. K(m) of Bgl3A was found to be 0.057 mM with p-nitrophenyl β-D-glucoside and V(max) was 1,920 U/mg. The purified enzyme exhibited glucose tolerance with a K(i) of 1.5 mM. Bgl3A readily hydrolyzed glucosides with β-linkage. Bgl3A activity was enhanced (156%) by Zn²⁺ and was not affected by other metal cations and reagents. The supplementation of Bgl3A (5 U/mg) with Trichoderma reesei cellulase complex (5 FPU/mg) resulted in about 70% of enhanced glucose production, which emphasizes the industrial importance of Bgl3A.
Links	PubMed Online version:10.1007/s12010-012-9645-4
Keywords	Biocatalysis; Carbon/metabolism; Cellulase/metabolism; Cellulose/metabolism; Fermentation; Hydrogen-Ion Concentration; Hydrolysis; Isoenzymes/biosynthesis; Isoenzymes/isolation & purification; Isoenzymes/metabolism; Penicillium/metabolism; Substrate Specificity; Temperature; Trichoderma/enzymology; beta-Glucosidase/biosynthesis; beta-Glucosidase/isolation & purification; beta-Glucosidase/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
TALFU:K4KB38		GO:0008422: beta-glucosidase activity	ECO:0000314:		F		Table 2 or 3 or 4	complete CACAO 8492
TALFU:K4KB38	enables	GO:0008422: beta-glucosidase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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