PMID:22389486

Citation	Bragg, J, Rajkovic, A, Anderson, C, Curtis, R, Van Houten, J, Begres, B, Naples, C, Snider, M, Fraga, D and Singer, M (2012) Identification and characterization of a putative arginine kinase homolog from Myxococcus xanthus required for fruiting body formation and cell differentiation. J. Bacteriol. 194:2668-76
Abstract	Arginine kinases catalyze the reversible transfer of a high-energy phosphoryl group from ATP to l-arginine to form phosphoarginine, which is used as an energy buffer in insects, crustaceans, and some unicellular organisms. It plays an analogous role to that of phosphocreatine in vertebrates. Recently, putative arginine kinases were identified in several bacterial species, including the social Gram-negative soil bacterium Myxococcus xanthus. It is still unclear what role these proteins play in bacteria and whether they have evolved to acquire novel functions in the species in which they are found. In this study, we biochemically purified and characterized a putative M. xanthus arginine kinase, Ark, and demonstrated that it has retained the ability to catalyze the phosphorylation of arginine by using ATP. We also constructed a null mutation in the ark gene and demonstrated its role in both certain stress responses and development.
Links	PubMed PMC3347173 Online version:10.1128/JB.06435-11
Keywords	Amino Acid Sequence; Arginine Kinase/chemistry; Arginine Kinase/metabolism; Gene Deletion; Gene Expression Regulation, Bacterial/physiology; Hydrogen Peroxide; Hydrogen-Ion Concentration; Molecular Sequence Data; Mutation; Myxococcus xanthus/cytology; Myxococcus xanthus/enzymology; Myxococcus xanthus/genetics; Myxococcus xanthus/metabolism; Phylogeny; Recombinant Proteins; Sodium Chloride; Stress, Physiological/drug effects
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MYXXD:Q1DA50		GO:0031155: regulation of fruiting body development	ECO:0000315:		P		"Surprisingly, development on CF agar was similar to that of the wild type, as the Δark mutant developed larger aggregates with slightly more defined edges than those for fruiting on TPM agar. The Δark mutant also developed a web-like appearance of less-defined aggregation on CF agar." "Under these conditions, we found that the Δark mutant produced 8.2% of the wild-type level of viable spores. The data are consistent with the hypothesis that ark is required under abrupt or stringent starvation conditions and plays less of a role in slow nutrient depletion." Figure 4	complete CACAO 8339
MYXXD:Q1DA50	involved_in	GO:0031155: regulation of reproductive fruiting body development	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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