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PMID:22361861
| Citation |
Leemhuis, H, Dijkman, WP, Dobruchowska, JM, Pijning, T, Grijpstra, P, Kralj, S, Kamerling, JP and Dijkhuizen, L (2012) 4,6-α-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily. ' ' |
|---|---|
| Abstract |
Family 70 glycoside hydrolase glucansucrase enzymes exclusively occur in lactic acid bacteria and synthesize a wide range of α-D: -glucan (abbreviated as α-glucan) oligo- and polysaccharides. Of the 47 characterized GH70 enzymes, 46 use sucrose as glucose donor. A single GH70 enzyme was recently found to be inactive with sucrose and to utilize maltooligosaccharides [(1→4)-α-D: -glucooligosaccharides] as glucose donor substrates for α-glucan synthesis, acting as a 4,6-α-glucanotransferase (4,6-αGT) enzyme. Here, we report the characterization of two further GH70 4,6-αGT enzymes, i.e., from Lactobacillus reuteri strains DSM 20016 and ML1, which use maltooligosaccharides as glucose donor. Both enzymes cleave α1→4 glycosidic linkages and add the released glucose moieties one by one to the non-reducing end of growing linear α-glucan chains via α1→6 glycosidic linkages (α1→4 to α1→6 transfer activity). In this way, they convert pure maltooligosaccharide substrates into linear α-glucan product mixtures with about 50% α1→6 glycosidic bonds (isomalto/maltooligosaccharides). These new α-glucan products may provide an exciting type of carbohydrate for the food industry. The results show that 4,6-αGTs occur more widespread in family GH70 and can be considered as a GH70 subfamily. Sequence analysis allowed identification of amino acid residues in acceptor substrate binding subsites +1 and +2, differing between GH70 GTF and 4,6-αGT enzymes. |
| Links |
PubMed Online version:10.1007/s00253-012-3943-1 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0046527: glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0046527: glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0004583: dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0009011: starch synthase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0016759: cellulose synthase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0032001: 1,4-alpha-glucan 6-alpha-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033556: dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033820: DNA alpha-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033821: DNA beta-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033822: glucosyl-DNA beta-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033823: procollagen glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033824: alternansucrase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033825: oligosaccharide 4-alpha-D-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033826: xyloglucan 4-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033828: glucosylglycerol-phosphate synthase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033831: kojibiose phosphorylase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
|
enables |
GO:0032001: 1,4-alpha-glucan 6-alpha-glucosyltransferase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0033825: oligosaccharide 4-alpha-D-glucosyltransferase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0032001: 1,4-alpha-glucan 6-alpha-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0033825: oligosaccharide 4-alpha-D-glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
| GO:0046527: glucosyltransferase activity |
ECO:0000314: |
F |
Figure 2, Figure 5 |
complete | ||||
|
enables |
GO:0032001: 1,4-alpha-glucan 6-alpha-glucosyltransferase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0033825: oligosaccharide 4-alpha-D-glucosyltransferase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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