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PMID:22320452

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Citation

Becherelli, M, Manetti, AG, Buccato, S, Viciani, E, Ciucchi, L, Mollica, G, Grandi, G and Margarit, I (2012) The ancillary protein 1 of Streptococcus pyogenes FCT-1 pili mediates cell adhesion and biofilm formation through heterophilic as well as homophilic interactions. Mol. Microbiol. 83:1035-47

Abstract

Gram-positive pili are known to play a role in bacterial adhesion to epithelial cells and in the formation of biofilm microbial communities. In the present study we undertook the functional characterization of the pilus ancillary protein 1 (AP1_M6) from Streptococcus pyogenes isolates expressing the FCT-1 pilus variant, known to be strong biofilm formers. Cell binding and biofilm formation assays using S. pyogenes in-frame deletion mutants, Lactococcus expressing heterologous FCT-1 pili and purified recombinant AP1_M6, indicated that this pilin is a strong cell adhesin that is also involved in bacterial biofilm formation. Moreover, we show that AP1_M6 establishes homophilic interactions that mediate inter-bacterial contact, possibly promoting bacterial colonization of target epithelial cells in the form of three-dimensional microcolonies. Finally, AP1_M6 knockout mutants were less virulent in mice, indicating that this protein is also implicated in GAS systemic infection.

Links

PubMed PMC3490378 Online version:10.1111/j.1365-2958.2012.07987.x

Keywords

Adhesins, Bacterial/genetics; Adhesins, Bacterial/metabolism; Animals; Bacterial Adhesion; Biofilms; Epithelial Cells/microbiology; Fimbriae Proteins/genetics; Fimbriae Proteins/metabolism; Fimbriae, Bacterial/metabolism; Mice; Sequence Deletion; Streptococcal Infections/microbiology; Streptococcus pyogenes/genetics; Streptococcus pyogenes/metabolism; Streptococcus pyogenes/pathogenicity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

STRPY:Q8RM89

GO:1900189: positive regulation of cell adhesion involved in single-species biofilm formation

ECO:0000315:

P

Figure 1D shows a signifcant decrease in A459 cells associated with ap1-knockout mutants compared to the wild-type. Figure 4, panels A and B, shows a significant drop in optical density in the wild-type compared to the knockout, implying more aggregation and biofilm formation.

complete
CACAO 10540

Notes

See also

References

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