PMID:22268462

Citation	Ruangkiattikul, N, Bhubhanil, S, Chamsing, J, Niamyim, P, Sukchawalit, R and Mongkolsuk, S (2012) Agrobacterium tumefaciens membrane-bound ferritin plays a role in protection against hydrogen peroxide toxicity and is negatively regulated by the iron response regulator. FEMS Microbiol. Lett. 329:87-92
Abstract	An Agrobacterium tumefaciens membrane-bound ferritin (mbfA) mutant was generated to assess the physiological functions of mbfA in response to iron and hydrogen peroxide (H(2) O(2) ) stresses. Wild-type and the mbfA mutant strains showed similar growth under high- and low-iron conditions. The mbfA mutant was more sensitive to H(2) O(2) than wild-type strain. Expression of a functional mbfA gene could complement the H(2) O(2) -hypersensitive phenotype of the mbfA mutant and a rhizobial iron regulator (rirA) mutant, suggesting that MbfA protects cells from H(2) O(2) toxicity by sequestering intracellular free iron, thus preventing the Fenton reaction. The expression of mbfA could be induced in response to iron and to H(2) O(2) treatment. The iron response regulator (irr) also acted as a repressor of mbfA expression. An irr mutant had high constitutive expression of mbfA, which partly contributed to the H(2) O(2) -hyperresistant phenotype of the irr mutant. The data reported here demonstrate an important role of A. tumefaciens MbfA in the cellular defence against iron and H(2) O(2) stresses.
Links	PubMed Online version:10.1111/j.1574-6968.2012.02509.x
Keywords	Agrobacterium tumefaciens/drug effects; Agrobacterium tumefaciens/growth & development; Agrobacterium tumefaciens/metabolism; Bacterial Proteins/metabolism; Ferritins/genetics; Ferritins/metabolism; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Genetic Complementation Test; Hydrogen Peroxide/metabolism; Hydrogen Peroxide/toxicity; Iron/metabolism; Iron/toxicity; Membrane Proteins/genetics; Membrane Proteins/metabolism; Transcription Factors/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
AGRFC:A9CKH9	Contributes to	GO:0006979: response to oxidative stress	ECO:0000315:		P		This is shown in figures 2, 3, and 4.	complete
AGRFC:A9CKH9	Contributes to	GO:0010039: response to iron ion	ECO:0000315:		P		This is shown in figure 4.	complete

References

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