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PMID:22232269
| Citation |
Sun, Y, Li, Q, Li, Z, Zhang, Y, Zhao, J and Wang, L (2012) Molecular cloning, expression, purification, and functional characterization of palustrin-2CE, an antimicrobial peptide of Rana chensinensis. Biosci. Biotechnol. Biochem. 76:157-62 |
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| Abstract |
Antimicrobial peptides are effector molecules of the innate immunity of amphibians. Here, one antimicrobial peptide cDNA precursor, prepropalustrin-2CE3, from the tadpole of the Chinese brown frog Rana chensinensis was cloned. The coding sequence corresponding to the mature palustrin-2CE peptide was subcloned into pGEX-6p-1. The soluble GST-palustrin-2CE fusion protein was successfully expressed in the BL21(DE3)pLysS strain at 16 °C, and the proportion of the fusion protein reached 35%-39% of the total cellular protein. After removal of the GST-fusion tag, the purity of the palustrin-2CE obtained by Sephadex G50 chromatography was about 97%. Moreover, the purified palustrin-2CE displayed obviously inhibitory activities against the sensitive bacteria Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli, and multi-drug resistant S. aureus and E. coli. These findings suggest that the tadpole of the Chinese brown frog is a unique source of antimicrobial peptides and indicates the therapeutic potential of the palustrin-2CE peptide. |
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| Keywords |
Amino Acid Sequence; Animals; Antimicrobial Cationic Peptides/chemistry; Antimicrobial Cationic Peptides/genetics; Antimicrobial Cationic Peptides/isolation & purification; Antimicrobial Cationic Peptides/pharmacology; Bacteria/drug effects; Bacteria/growth & development; Base Sequence; Cloning, Molecular; DNA, Complementary/genetics; Gene Expression; Larva/genetics; Molecular Sequence Data; Ranidae/genetics |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0050829: defense response to Gram-negative bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
See also
References
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