PMID:22174625

Citation	Xu, DK, Jin, QJ, Xie, YJ, Liu, YH, Lin, YT, Shen, WB and Zhou, YJ (2011) Characterization of a wheat heme oxygenase-1 gene and its responses to different abiotic stresses. Int J Mol Sci 12:7692-707
Abstract	In animals and recently in plants, heme oxygenase-1 (HO1) has been found to confer protection against a variety of oxidant-induced cell and tissue injuries. In this study, a wheat (Triticum aestivum) HO1 gene TaHO1 was cloned and sequenced. It encodes a polypeptide of 31.7 kD with a putative N-terminal plastid transit peptide. The amino acid sequence of TaHO1 was found to be 78% similar to that of maize HO1. Phylogenetic analysis revealed that TaHO1 clusters together with the HO1-like sequences in plants. The purified recombinant TaHO1 protein expressed in Escherichia coli was active in the conversion of heme to biliverdin IXa (BV), and showed that the V(max) was 8.8 U·mg(-1) protein with an apparent K(m) value for hemin of 3.04 μM. The optimum Tm and pH were 35 °C and 7.4, respectively. The result of subcellular localization of TaHO1 showed that the putative transit peptide was sufficient for green fluorescent protein (GFP) to localize in chloroplast and implied that TaHO1 gene product is at least localized in the chloroplast. Moreover, we found that TaHO1 mRNA could be differentially induced by the well-known nitric oxide (NO) donor sodium nitroprusside (SNP), gibberellin acid (GA), abscisic acid (ABA), hydrogen peroxide (H(2)O(2)) and NaCl treatments. Therefore, the results suggested that TaHO1 might play an important role in abiotic stress responses.
Links	PubMed PMC3233431 Online version:10.3390/ijms12117692
Keywords
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
WHEAT:D6QXI6	part_of	GO:0009536: plastid	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
WHEAT:D6QXI6		GO:0009536: plastid	ECO:0000314:		C		Figure 5	complete CACAO 8224

References

See Help:References for how to manage references in GONUTS.