PMID:22140501

Citation	Lampert, TJ, Coleman, KD and Hennessey, TM (2011) A knockout mutation of a constitutive GPCR in Tetrahymena decreases both G-protein activity and chemoattraction. PLoS ONE 6:e28022
Abstract	Although G-protein coupled receptors (GPCRs) are a common element in many chemosensory transduction pathways in eukaryotic cells, no GPCR or regulated G-protein activity has yet been shown in any ciliate. To study the possible role for a GPCR in the chemoresponses of the ciliate Tetrahymena, we have generated a number of macronuclear gene knockouts of putative GPCRs found in the Tetrahymena Genome database. One of these knockout mutants, called G6, is a complete knockout of a gene that we call GPCR6 (TTHERM_00925490). Based on sequence comparisons, the Gpcr6p protein belongs to the Rhodopsin Family of GPCRs. Notably, Gpcr6p shares highest amino acid sequence homologies to GPCRs from Paramecium and several plants. One of the phenotypes of the G6 mutant is a decreased responsiveness to the depolarizing ions Ba²⁺ and K⁺, suggesting a decrease in basal excitability (decrease in Ca²⁺ channel activity). The other major phenotype of G6 is a loss of chemoattraction to lysophosphatidic acid (LPA) and proteose peptone (PP), two known chemoattractants in Tetrahymena. Using microsomal [³⁵S]GTPγS binding assays, we found that wild-type (CU427) have a prominent basal G-protein activity. This activity is decreased to the same level by pertussis toxin (a G-protein inhibitor), addition of chemoattractants, or the G6 mutant. Since the basal G-protein activity is decreased by the GPCR6 knockout, it is likely that this gene codes for a constitutively active GPCR in Tetrahymena. We propose that chemoattractants like LPA and PP cause attraction in Tetrahymena by decreasing the basal G-protein stimulating activity of Gpcr6p. This leads to decreased excitability in wild-type and longer runs of smooth forward swimming (less interrupted by direction changes) towards the attractant. Therefore, these attractants may work as inverse agonists through the constitutively active Gpcr6p coupled to a pertussis-sensitive G-protein.
Links	PubMed PMC3226668 Online version:10.1371/journal.pone.0028022
Keywords	Amino Acid Sequence; Cell Line; Chemotaxis/drug effects; Electrophysiological Phenomena/drug effects; GTP-Binding Proteins/metabolism; Gene Knockout Techniques; Genome/genetics; Microsomes/drug effects; Microsomes/metabolism; Models, Biological; Molecular Sequence Data; Mutation/genetics; Pertussis Toxin/pharmacology; Protozoan Proteins/chemistry; Protozoan Proteins/metabolism; Receptors, G-Protein-Coupled/chemistry; Receptors, G-Protein-Coupled/genetics; Reproducibility of Results; Sequence Analysis, Protein; Signal Transduction/drug effects; Tetrahymena/cytology; Tetrahymena/drug effects; Tetrahymena/genetics
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