PMID:22140229

Citation	Li, Z, Pan, G, Li, T, Huang, W, Chen, J, Geng, L, Yang, D, Wang, L and Zhou, Z (2012) SWP5, a spore wall protein, interacts with polar tube proteins in the parasitic microsporidian Nosema bombycis. Eukaryotic Cell 11:229-37
Abstract	Microsporidia are a group of eukaryotic intracellular parasites that infect almost all vertebrates and invertebrates. The microsporidian invasion process involves the extrusion of a unique polar tube into host cells. Both the spore wall and the polar tube play an important role in microsporidian pathogenesis. So far, five spore wall proteins (SWP1, SWP2, Enp1, Enp2, and EcCDA) from Encephalitozoon intestinalis and Encephalitozoon cuniculi and five spore wall proteins (SWP32, SWP30, SWP26, SWP25, and NbSWP5) from the silkworm pathogen Nosema bombycis have been identified. Here we report the identification and characterization of a spore wall protein (SWP5) with a molecular mass of 20.3 kDa in N. bombycis. This protein has low sequence similarity to other eukaryotic proteins. Immunolocalization analysis showed SWP5 localized to the exospore and the region of the polar tube in mature spores. Immunoprecipitation, mass spectrometry, and immunofluorescence analyses revealed that SWP5 interacts with the polar tube proteins PTP2 and PTP3. Anti-SWP5 serum pretreatment of mature spores significantly decreased their polar tube extrusion rate. Taken together, our results show that SWP5 is a spore wall protein localized to the spore wall and that it interacts with the polar tube, may play an important role in supporting the structural integrity of the spore wall, and potentially modulates the course of infection of N. bombycis.
Links	PubMed PMC3272902 Online version:10.1128/EC.05127-11
Keywords	Cell Wall/chemistry; Cell Wall/metabolism; Fluorescent Antibody Technique; Fungal Proteins/chemistry; Fungal Proteins/metabolism; Molecular Weight; Nosema/growth & development; Spores, Fungal/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NOSBO:G0Z414		GO:0031160: spore wall	ECO:0000314:		C		Fig 4b: spores were incubated with SWP5 antiserum, green fluorescence was observed Fig 4c: spores were treated with SDS (known to remove antigens from surface of mature spores) and incubated in SWP5 antiserum, no fluorescence was observed	complete CACAO 10236
NOSBO:G0Z414	part_of	GO:0031160: spore wall	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
NOSBO:B3STN9		GO:0031160: spore wall	ECO:0000314:		C		Fig 5: spores were incubated with SWP5 antibody, a secondary antibody was conjugated with gold, gold particles were found on exospore (outer spore wall) of spores	complete CACAO 10275

References

See Help:References for how to manage references in GONUTS.