PMID:22046131

Citation	Amari, K, Lerich, A, Schmitt-Keichinger, C, Dolja, VV and Ritzenthaler, C (2011) Tubule-guided cell-to-cell movement of a plant virus requires class XI myosin motors. PLoS Pathog. 7:e1002327
Abstract	Cell-to-cell movement of plant viruses occurs via plasmodesmata (PD), organelles that evolved to facilitate intercellular communications. Viral movement proteins (MP) modify PD to allow passage of the virus particles or nucleoproteins. This passage occurs via several distinct mechanisms one of which is MP-dependent formation of the tubules that traverse PD and provide a conduit for virion translocation. The MP of tubule-forming viruses including Grapevine fanleaf virus (GFLV) recruit the plant PD receptors called Plasmodesmata Located Proteins (PDLP) to mediate tubule assembly and virus movement. Here we show that PDLP1 is transported to PD through a specific route within the secretory pathway in a myosin-dependent manner. This transport relies primarily on the class XI myosins XI-K and XI-2. Inactivation of these myosins using dominant negative inhibition results in mislocalization of PDLP and MP and suppression of GFLV movement. We also found that the proper targeting of specific markers of the Golgi apparatus, the plasma membrane, PD, lipid raft subdomains within the plasma membrane, and the tonoplast was not affected by myosin XI-K inhibition. However, the normal tonoplast dynamics required myosin XI-K activity. These results reveal a new pathway of the myosin-dependent protein trafficking to PD that is hijacked by GFLV to promote tubule-guided transport of this virus between plant cells.
Links	PubMed PMC3203191 Online version:10.1371/journal.ppat.1002327
Keywords	Bicyclo Compounds, Heterocyclic/pharmacology; Golgi Apparatus/drug effects; Golgi Apparatus/physiology; Golgi Apparatus/virology; Host-Pathogen Interactions; Membrane Microdomains/drug effects; Membrane Microdomains/virology; Microtubules/drug effects; Microtubules/physiology; Microtubules/virology; Myosins/antagonists & inhibitors; Myosins/metabolism; Nepovirus/drug effects; Nepovirus/pathogenicity; Nepovirus/physiology; Plant Viral Movement Proteins/physiology; Protein Transport/drug effects; Protein Transport/physiology; Thiazolidines/pharmacology; Viral Nonstructural Proteins
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NICBE:B0CN61		GO:0046740: spread of virus in host, cell to cell	ECO:0000315:		P		Fig. 1 (B,C,D)	complete CACAO 2612
NICBE:B0CN61	involved_in	GO:0046740: transport of virus in host, cell to cell	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
NICBE:B0CN60	involved_in	GO:0046740: transport of virus in host, cell to cell	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
NICBE:B0CN60		GO:0046740: spread of virus in host, cell to cell	ECO:0000315:		P		Fig. 1 (B,C,D)	complete CACAO 2608
NICBE:B0CN59	involved_in	GO:0046740: transport of virus in host, cell to cell	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
NICBE:B0CN59		GO:0046740: spread of virus in host, cell to cell	ECO:0000315:		P		Fig. 1 (B,C,D)	complete CACAO 2609

References

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