PMID:22039214

Citation	Liu, W, Kohlen, W, Lillo, A, Op den Camp, R, Ivanov, S, Hartog, M, Limpens, E, Jamil, M, Smaczniak, C, Kaufmann, K, Yang, WC, Hooiveld, GJ, Charnikhova, T, Bouwmeester, HJ, Bisseling, T and Geurts, R (2011) Strigolactone biosynthesis in Medicago truncatula and rice requires the symbiotic GRAS-type transcription factors NSP1 and NSP2. Plant Cell 23:3853-65
Abstract	Legume GRAS (GAI, RGA, SCR)-type transcription factors NODULATION SIGNALING PATHWAY1 (NSP1) and NSP2 are essential for rhizobium Nod factor-induced nodulation. Both proteins are considered to be Nod factor response factors regulating gene expression after symbiotic signaling. However, legume NSP1 and NSP2 can be functionally replaced by nonlegume orthologs, including rice (Oryza sativa) NSP1 and NSP2, indicating that both proteins are functionally conserved in higher plants. Here, we show that NSP1 and NSP2 are indispensable for strigolactone (SL) biosynthesis in the legume Medicago truncatula and in rice. Mutant nsp1 plants do not produce SLs, whereas in M. truncatula, NSP2 is essential for conversion of orobanchol into didehydro-orobanchol, which is the main SL produced by this species. The disturbed SL biosynthesis in nsp1 nsp2 mutant backgrounds correlates with reduced expression of DWARF27, a gene essential for SL biosynthesis. Rice and M. truncatula represent distinct phylogenetic lineages that split approximately 150 million years ago. Therefore, we conclude that regulation of SL biosynthesis by NSP1 and NSP2 is an ancestral function conserved in higher plants. NSP1 and NSP2 are single-copy genes in legumes, which implies that both proteins fulfill dual regulatory functions to control downstream targets after rhizobium-induced signaling as well as SL biosynthesis in nonsymbiotic conditions.
Links	PubMed PMC3229154 Online version:10.1105/tpc.111.089771
Keywords	Amino Acid Sequence; Carotenoids/analysis; Carotenoids/metabolism; Down-Regulation; Gene Expression Profiling; Gene Expression Regulation, Plant; Lactones/analysis; Lactones/chemistry; Lactones/metabolism; Medicago truncatula/genetics; Medicago truncatula/growth & development; Medicago truncatula/microbiology; Medicago truncatula/physiology; Molecular Sequence Data; Mutation; Oligonucleotide Array Sequence Analysis; Oryza sativa/genetics; Oryza sativa/growth & development; Oryza sativa/microbiology; Oryza sativa/physiology; Phenotype; Phylogeny; Plant Growth Regulators/metabolism; Plant Proteins/genetics; Plant Proteins/metabolism; Plant Root Nodulation; Plant Roots/genetics; Plant Roots/growth & development; Plant Roots/microbiology; Plant Roots/physiology; Sesquiterpenes/metabolism; Signal Transduction; Sinorhizobium meliloti/physiology; Symbiosis; Transcription Factors/genetics; Transcription Factors/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ORYSJ:Q84MQ9		GO:0042446: hormone biosynthetic process	ECO:0000315:		P		Figure 5 shows that mutants that have >90% knock down in NSP1 have reduced strigolactone (a plant hormome) production.	complete CACAO 5474
ORYSJ:Q84MQ9		GO:2000032: regulation of secondary shoot formation	ECO:0000315:		P		Figure 5c shows that plants that had a >90% knockdown in NSP1 had an increased number of tillers (secondary shoots).	complete CACAO 5475
ORYSJ:Q84MQ9	involved_in	GO:0042446: hormone biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ORYSJ:Q84MQ9	involved_in	GO:2000032: regulation of secondary shoot formation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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