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PMID:21866344

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Citation

Li, G, Dong, G, Li, B, Li, Q, Kronzucker, HJ and Shi, W (2012) Isolation and characterization of a novel ammonium overly sensitive mutant, amos2, in Arabidopsis thaliana. Planta 235:239-52

Abstract

Ammonium (NH(4)(+)) toxicity is a significant agricultural problem globally, compromising crop growth and productivity in many areas. However, the molecular mechanisms of NH(4)(+) toxicity are still poorly understood, in part due to a lack of valuable genetic resources. Here, a novel Arabidopsis mutant, amos2 (ammonium overly sensitive 2), displaying hypersensitivity to NH(4) (+) in both shoots and roots, was isolated. The mutant exhibits the hallmarks of NH(4)(+) toxicity at significantly elevated levels: severely suppressed shoot biomass, increased leaf chlorosis, and inhibition of lateral root formation. Amos2 hypersensitivity is associated with excessive NH(4)(+) accumulation in shoots and a reduction in tissue potassium (K(+)), calcium (Ca(2+)), and magnesium (Mg(2+)). We show that the lesion is specific to the NH(4)(+) ion, is independent of NH(4)(+) metabolism, and can be partially rescued by elevated external K(+). The amos2 lesion was mapped to a 16-cM interval on top of chromosome 1, where no similar mutation has been previously mapped. Our study identifies a novel locus controlling cation homeostasis under NH(4)(+) stress and provides a tool for the future identification of critical genes involved in the development of NH(4)(+) toxicity.

Links

PubMed Online version:10.1007/s00425-011-1504-y

Keywords

Ammonium Sulfate/metabolism; Ammonium Sulfate/pharmacology; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Calcium/metabolism; Chromosome Mapping; Chromosomes, Plant/genetics; Crosses, Genetic; Culture Media/metabolism; Genes, Plant; Homeostasis; Magnesium/metabolism; Mutation; Phenotype; Plant Diseases/chemically induced; Plant Roots/drug effects; Plant Roots/growth & development; Plant Roots/metabolism; Plant Shoots/drug effects; Plant Shoots/growth & development; Plant Shoots/metabolism; Potassium/metabolism; Potassium/pharmacology; Quaternary Ammonium Compounds/metabolism; Species Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status


See also

References

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