PMID:21858144

Citation	Wu, MC, Lin, TL, Hsieh, PF, Yang, HC and Wang, JT (2011) Isolation of genes involved in biofilm formation of a Klebsiella pneumoniae strain causing pyogenic liver abscess. PLoS ONE 6:e23500
Abstract	Community-acquired pyogenic liver abscess (PLA) complicated with meningitis and endophthalmitis caused by Klebsiella pneumoniae is an emerging infectious disease. To investigate the mechanisms and effects of biofilm formation of K. pneumoniae causing PLA, microtiter plate assays were used to determine the levels of biofilm formed by K. pneumoniae clinical isolates and to screen for biofilm-altered mutants from a transposon mutant library of a K. pneumoniae PLA-associated strain.
Links	PubMed PMC3155550 Online version:10.1371/journal.pone.0023500
Keywords	Animals; Bacterial Capsules/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Biofilms/growth & development; Disaccharidases/genetics; Disaccharidases/isolation & purification; Disaccharidases/metabolism; Female; Gene Expression Regulation, Bacterial; Humans; Klebsiella Infections/microbiology; Klebsiella pneumoniae/genetics; Klebsiella pneumoniae/physiology; Liver Abscess, Pyogenic/microbiology; Male; Membrane Proteins/genetics; Membrane Proteins/isolation & purification; Membrane Proteins/metabolism; Mice; Mice, Inbred BALB C; Mutation; Reverse Transcriptase Polymerase Chain Reaction; Trehalose/metabolism
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Significance

Gene product	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
KLEPN:C4X1I2	GO:0042710: biofilm formation	IMP: Inferred from Mutant Phenotype:	P	Fig. 2	complete
KLEPN:C4X1I2	GO:0045227: capsule polysaccharide biosynthetic process	IMP: Inferred from Mutant Phenotype:	P	Fig. 3	complete
KLEPN:B6ZN41	GO:0042710: biofilm formation	ECO:0000314:		P		figure 3: Shows that the sample with the sugE protein has a thicker biofilm than the WT.	complete

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