PMID:21801253

Citation	Zhang, B, Chen, HW, Mu, RL, Zhang, WK, Zhao, MY, Wei, W, Wang, F, Yu, H, Lei, G, Zou, HF, Ma, B, Chen, SY and Zhang, JS (2011) NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis. Plant J. 68:830-43
Abstract	The NIMA-related kinases (NEKs) are a family of serine/threonine kinases involved largely in cell cycle control in fungi, mammals and other eukaryotes. In Arabidopsis, NEK6 is involved in the regulation of epidermal cell morphogenesis. However, other roles of NEK6 in plants are less well understood. Here we report functions of NEK6 in plant growth, development and stress responses in Arabidopsis. NEK6 transcripts and proteins are induced by ethylene precursor ACC and salt stress. Expression of other NEK genes except NEK5 is also responsive to the two treatments. Overexpression and mutant analysis disclose that the NEK6 gene increases rosette growth, seed yield and lateral root formation. However, NEK6 appears to play a negative role in the control of seed size. The gene also promotes plant tolerance to salt stress and osmotic stress in its overexpressing plants. The NEK6 gene may achieve its function through suppression of ethylene biosynthesis and activation of CYCB1;1 and CYCA3;1 expression. Our present study reveals new functions of the NEK6 gene in plant growth and stress tolerance, and manipulation of NEK6 may improve important agronomic traits in crop plants.
Links	PubMed Online version:10.1111/j.1365-313X.2011.04733.x
Keywords	Arabidopsis/drug effects; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Ethylenes/metabolism; Gene Expression Regulation, Plant; Mannitol/pharmacology; Mutagenesis, Insertional; Osmotic Pressure; Phenotype; Plant Leaves/genetics; Plant Leaves/metabolism; Plant Leaves/ultrastructure; Plant Roots/drug effects; Plant Roots/genetics; Plant Roots/growth & development; Plant Roots/metabolism; Plants, Genetically Modified/drug effects; Plants, Genetically Modified/enzymology; Plants, Genetically Modified/genetics; Plants, Genetically Modified/growth & development; Protein Kinases/genetics; Protein Kinases/metabolism; Salt-Tolerant Plants/drug effects; Salt-Tolerant Plants/enzymology; Salt-Tolerant Plants/genetics; Salt-Tolerant Plants/growth & development; Seeds/genetics; Seeds/metabolism; Seeds/ultrastructure; Sodium Chloride/pharmacology; Stress, Physiological
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:NEK6		GO:0040008: regulation of growth	ECO:0000315:		P		Figure 2	complete CACAO 5911
ARATH:NEK6		GO:0010311: lateral root formation	ECO:0000315:		P		Figure 5	complete CACAO 5915
ARATH:NEK6		GO:1901000: positive regulation of response to salt stress	ECO:0000315:		P		Figure 6 shows the growth and survival rate of mutant and wild-type plants after treatment with NaCl.	complete CACAO 5916
ARATH:NEK6		GO:0047484: regulation of response to osmotic stress	ECO:0000315:		P		Figure 7 shows the growth and survival of mutant and wild-type plants.	complete CACAO 5917
ARATH:NEK6		GO:0000079: positive regulation of cyclin-dependent protein kinase activity	ECO:0000315:		P		Figure 8	complete CACAO 5920
ARATH:NEK6		GO:0010366: negative regulation of ethylene biosynthetic process	ECO:0000315:		P		Figure 9	complete CACAO 5921
ARATH:NEK6	involved_in	GO:0010366: negative regulation of ethylene biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:NEK6	involved_in	GO:0010311: lateral root formation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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