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PMID:21771526

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Citation

Tanaka, T, Kawano, S, Nakao, S, Umemiya-Shirafuji, R, Rahman, MM, Boldbaatar, D, Battur, B, Liao, M and Fujisaki, K (2010) The identification and characterization of lysozyme from the hard tick Haemaphysalis longicornis. Ticks Tick Borne Dis 1:178-85

Abstract

A full-length cDNA-encoding lysozyme was obtained from cDNA libraries of salivary glands of the hard tick Haemaphysalis longicornis and designated as HlLysozyme. The HlLysozyme sequence represents an open reading frame for a putative signal peptide and the mature protein composed of 121 amino acids. The calculated molecular weight of the protein is 13.7 kDa, and the theoretical isoelectric point is 9.85. HlLysozyme shares 41-79% amino acid sequence identity with the lysozymes of other organisms. The activity of recombinant HlLysozyme expressed in Escherichia coli was confirmed by a lytic zone assay using lyophilized Micrococcus lysodeikticus. The HlLysozyme activity decreased at 70 °C and was demonstrated at acidic side and neutral in a pH range. Elevated gene expression of HlLysozyme was observed when female ticks were challenged with bacteria, suggesting possible roles of lysozyme as an innate immunity of ticks against microorganisms.

Links

PubMed Online version:10.1016/j.ttbdis.2010.09.001

Keywords

Amino Acid Sequence; Animals; Base Sequence; DNA, Complementary/chemistry; Escherichia coli/immunology; Feeding Behavior; Female; Ixodidae/enzymology; Molecular Sequence Data; Muramidase/genetics; Muramidase/isolation & purification; Muramidase/metabolism; Staphylococcus aureus/immunology; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HAELO:F2Z7L0

GO:0003796: lysozyme activity

ECO:0000314:

F

Figure 4 shows the activity of recombinant HILysozyme, that was performed using Micrococcus lysodeikticus powder as a substrate. It is shown that the diameter of the clear zone depends on the protein concentration. This effect is similar to the effect caused by the egg white lysozyme used as control. (Although this data were not shown)

complete
CACAO 4277

HAELO:F2Z7L0

enables

GO:0003796: lysozyme activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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