PMID:21750256

Citation	Wright, SJ, Inchausti, R, Eaton, CJ, Krystofova, S and Borkovich, KA (2011) RIC8 is a guanine-nucleotide exchange factor for Galpha subunits that regulates growth and development in Neurospora crassa. Genetics 189:165-76
Abstract	Heterotrimeric (αβγ) G proteins are crucial components of eukaryotic signal transduction pathways. G-protein-coupled receptors (GPCRs) act as guanine nucleotide exchange factors (GEFs) for Gα subunits. Recently, facilitated GDP/GTP exchange by non-GPCR GEFs, such as RIC8, has emerged as an important mechanism for Gα regulation in animals. RIC8 is present in animals and filamentous fungi, such as the model eukaryote Neurospora crassa, but is absent from the genomes of baker's yeast and plants. In Neurospora, deletion of ric8 leads to profound defects in growth and asexual and sexual development, similar to those observed for a mutant lacking the Gα genes gna-1 and gna-3. In addition, constitutively activated alleles of gna-1 and gna-3 rescue many defects of Δric8 mutants. Similar to reports in Drosophila, Neurospora Δric8 strains have greatly reduced levels of G-protein subunits. Effects on cAMP signaling are suggested by low levels of adenylyl cyclase protein in Δric8 mutants and suppression of Δric8 by a mutation in the protein kinase A regulatory subunit. RIC8 acts as a GEF for GNA-1 and GNA-3 in vitro, with the strongest effect on GNA-3. Our results support a role for RIC8 in regulating GNA-1 and GNA-3 in Neurospora.
Links	PubMed PMC3176126 Online version:10.1534/genetics.111.129270
Keywords	Amino Acid Sequence; Cyclic AMP/metabolism; Cytoplasm/metabolism; Epistasis, Genetic; Fungal Proteins/genetics; Fungal Proteins/isolation & purification; Fungal Proteins/metabolism; GTP-Binding Protein alpha Subunits/genetics; GTP-Binding Protein alpha Subunits/metabolism; Guanine Nucleotide Exchange Factors/genetics; Guanine Nucleotide Exchange Factors/isolation & purification; Guanine Nucleotide Exchange Factors/metabolism; Guanosine/metabolism; Hyphae/metabolism; Molecular Sequence Data; Neurospora crassa/genetics; Neurospora crassa/growth & development; Neurospora crassa/metabolism; Protein Transport; Sequence Alignment; Signal Transduction; Spores, Fungal/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NEUCR:Q7SD74	part_of	GO:0005829: cytosol	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
NEUCR:Q7SD74		GO:0005829: cytosol	ECO:0000314:		C		Figure 4	complete CACAO 8267

References

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