PMID:21706164

Citation	Thabet, I, Guirimand, G, Guihur, A, Lanoue, A, Courdavault, V, Papon, N, Bouzid, S, Giglioli-Guivarc'h, N, Simkin, AJ and Clastre, M (2012) Characterization and subcellular localization of geranylgeranyl diphosphate synthase from Catharanthus roseus. Mol. Biol. Rep. 39:3235-43
Abstract	The enzyme geranylgeranyl diphosphate synthase (GGPS: EC 2.5.1.1, EC 2.5.1.10, EC 2.5.1.29) catalyses the formation of geranylgeranyl diphosphate (GGPP) from isopentenyl diphosphate and dimethylallyl diphosphate via three successive condensation reactions. A full-length nucleotide sequence of GGPS (named CrGGPS) was cloned from the medicinal plant Catharanthus roseus. The deduced polypeptide has 383 amino acids with a calculated mass of 41.6 kDa and possesses prenyltransferase signatures characteristic of plant type II GGPS. The enzyme was characterized by functional complementation in carotenoid accumulating strains of Escherichia coli. When cultures of Catharanthus cell lines were treated with methyljasmonate, no specific increase in transcript levels were observed. In plants, GGPS are encoded by a small multigene family and the isoforms have been shown to be localized in three different subcellular compartments: chloroplast, endoplasmic reticulum and mitochondria. We investigated the subcellular distribution of CrGGPS through transient transformations of C. roseus cells with a yellow fluorescent protein-fused construct. Our results clearly indicate that CrGGPS is located to plastids within stroma and stromules.
Links	PubMed Online version:10.1007/s11033-011-1091-9
Keywords	Acetates; Amino Acid Sequence; Bacterial Proteins; Base Sequence; Catharanthus/enzymology; Chromatography, High Pressure Liquid; Cloning, Molecular; Cyclopentanes; DNA Primers/genetics; DNA, Complementary/biosynthesis; Escherichia coli; Farnesyltranstransferase/genetics; Farnesyltranstransferase/metabolism; Genetic Complementation Test; Intracellular Space/metabolism; Luminescent Proteins; Microscopy, Fluorescence; Molecular Sequence Data; Oxylipins; Plastids/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CATRO:F8UR58	part_of	GO:0010319: stromule	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
CATRO:F8UR58		GO:0010319: stromule	ECO:0000314:		C		Figure 4.	complete CACAO 8472

References

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