GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:21705681
| Citation |
Stephens, NA and Hajduk, SL (2011) Endosomal localization of the serum resistance-associated protein in African trypanosomes confers human infectivity. Eukaryotic Cell 10:1023-33 |
|---|---|
| Abstract |
Trypanosoma brucei rhodesiense is the causative agent of human African sleeping sickness. While the closely related subspecies T. brucei brucei is highly susceptible to lysis by a subclass of human high-density lipoproteins (HDL) called trypanosome lytic factor (TLF), T. brucei rhodesiense is resistant and therefore able to establish acute and fatal infections in humans. This resistance is due to expression of the serum resistance-associated (SRA) gene, a member of the variant surface glycoprotein (VSG) gene family. Although much has been done to establish the role of SRA in human serum resistance, the specific molecular mechanism of SRA-mediated resistance remains a mystery. Thus, we report the trafficking and steady-state localization of SRA in order to provide more insight into the mechanism of SRA-mediated resistance. We show that SRA traffics to the flagellar pocket of bloodstream-form T. brucei organisms, where it localizes transiently before being endocytosed to its steady-state localization in endosomes, and we demonstrate that the critical point of colocalization between SRA and TLF occurs intracellularly. |
| Links |
PubMed PMC3165451 Online version:10.1128/EC.05112-11 |
| Keywords |
Cells, Cultured; Endosomes/metabolism; Flagella/metabolism; Humans; Immune Evasion; Lipoproteins, HDL/metabolism; Membrane Glycoproteins/metabolism; Protein Binding; Protein Structure, Tertiary; Protein Transport; Protozoan Proteins/metabolism; Trypanosoma brucei rhodesiense/metabolism; Trypanosoma brucei rhodesiense/physiology; Trypanosomiasis, African/immunology; Trypanosomiasis, African/parasitology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0020016: flagellar pocket |
ECO:0000314: |
C |
Figure 1 exhibits the trafficking of SRA to the flagellar pocket using fluorescent staining. |
complete | ||||
|
part_of |
GO:0020016: ciliary pocket |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
