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PMID:21705642
| Citation |
Shi, J, Tan, H, Yu, XH, Liu, Y, Liang, W, Ranathunge, K, Franke, RB, Schreiber, L, Wang, Y, Kai, G, Shanklin, J, Ma, H and Zhang, D (2011) Defective pollen wall is required for anther and microspore development in rice and encodes a fatty acyl carrier protein reductase. Plant Cell 23:2225-46 |
|---|---|
| Abstract |
Aliphatic alcohols naturally exist in many organisms as important cellular components; however, their roles in extracellular polymer biosynthesis are poorly defined. We report here the isolation and characterization of a rice (Oryza sativa) male-sterile mutant, defective pollen wall (dpw), which displays defective anther development and degenerated pollen grains with an irregular exine. Chemical analysis revealed that dpw anthers had a dramatic reduction in cutin monomers and an altered composition of cuticular wax, as well as soluble fatty acids and alcohols. Using map-based cloning, we identified the DPW gene, which is expressed in both tapetal cells and microspores during anther development. Biochemical analysis of the recombinant DPW enzyme shows that it is a novel fatty acid reductase that produces 1-hexadecanol and exhibits >270-fold higher specificity for palmiltoyl-acyl carrier protein than for C16:0 CoA substrates. DPW was predominantly targeted to plastids mediated by its N-terminal transit peptide. Moreover, we demonstrate that the monocot DPW from rice complements the dicot Arabidopsis thaliana male sterile2 (ms2) mutant and is the probable ortholog of MS2. These data suggest that DPWs participate in a conserved step in primary fatty alcohol synthesis for anther cuticle and pollen sporopollenin biosynthesis in monocots and dicots. |
| Links |
PubMed PMC3160036 Online version:10.1105/tpc.111.087528 |
| Keywords |
Acyl Carrier Protein/genetics; Acyl Carrier Protein/metabolism; Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Fatty Alcohols/chemistry; Fatty Alcohols/metabolism; Flowers/chemistry; Flowers/enzymology; Flowers/growth & development; Flowers/ultrastructure; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genetic Complementation Test; Molecular Sequence Data; Molecular Structure; Mutation; Oryza sativa/anatomy & histology; Oryza sativa/enzymology; Oryza sativa/genetics; Oryza sativa/growth & development; Oxidoreductases/classification; Oxidoreductases/genetics; Oxidoreductases/metabolism; Phenotype; Phylogeny; Plant Proteins/classification; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Genetically Modified; Pollen/enzymology; Pollen/growth & development; Pollen/ultrastructure; Tissue Distribution |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0080019: fatty-acyl-CoA reductase (alcohol-forming) activity |
ECO:0000314: |
F |
Figure 9C and D show that purified DPW can reduce palmitoyl CoA and palmitoleoyl CoA into alcohols. |
complete | ||||
| GO:0010584: pollen exine formation |
ECO:0000315: |
P |
Figure 3 S vs T show the mutant dpw plants have abnormal pollen exine formation compared to the wildtype. |
complete | ||||
| GO:0009507: chloroplast |
ECO:0000314: |
C |
Figure 8A-D show GFP tagged DPW colocalizes with chloroplasts. |
complete | ||||
|
enables |
GO:0080019: fatty-acyl-CoA reductase (alcohol-forming) activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010584: pollen exine formation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0009507: chloroplast |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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