PMID:21617349

Citation	Kwon, GH, Park, JY, Kim, JS, Lim, J, Park, CS, Kwon, DY and Kim, JH (2011) Cloning and expression of a bpr gene encoding Bacillopeptidase F from Bacillus amyloliquefaciens CH86-1. J. Microbiol. Biotechnol. 21:515-8
Abstract	A gene encoding bacillopeptidase F, bpr86-1, was cloned from B. amyloliquefaciens CH86-1 isolated from cheonggukjang. This gene could encode a preproenzyme of 1,431 amino acids. When bpr86-1 was introduced into B. subtilis WB600 via pHY300PLK, an E. coli-Bacillus shuttle vector, the transformant showed fibrinolytic activity. During growth on LB, the fibrinolytic activity of cells increased sharply when they entered the stationary phase. The highest activity (761.4 mU/mg protein) was observed at 96 h of cultivation.
Links	PubMed
Keywords	Amino Acid Sequence; Bacillus/chemistry; Bacillus/enzymology; Bacillus/genetics; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cloning, Molecular; Gene Expression; Kinetics; Molecular Sequence Data; Sequence Alignment; Serine Endopeptidases/chemistry; Serine Endopeptidases/genetics; Serine Endopeptidases/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACAM:D2D3C3		GO:0008233: peptidase activity	ECO:0000314:		F		Direct Assay Evidence Code was inferred based on Figure 3: SDS-PAGE (left) and fibrin zymogram (right) of culture supernatant, which shows bands of 90, 55, and 40 kDa of B. subtilis TF (cells harboring pHYbpr86-1) that were likely derived from bacillopeptidse F.	complete CACAO 4372
BACAM:D2D3C3	enables	GO:0008233: peptidase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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