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PMID:21515767
| Citation |
Tran, NT, Den Hengst, CD, Gomez-Escribano, JP and Buttner, MJ (2011) Identification and characterization of CdgB, a diguanylate cyclase involved in developmental processes in Streptomyces coelicolor. J. Bacteriol. 193:3100-8 |
|---|---|
| Abstract |
We describe the identification and functional characterization of cdgB (SCO4281), a recently discovered target of BldD, a key regulator of morphological differentiation and antibiotic production in the mycelial bacteria of the genus Streptomyces. cdgB (cyclic dimeric GMP [c-di-GMP] B) encodes a GGDEF-containing protein that has diguanylate cyclase activity in vitro. Consistent with this enzymatic activity, heterologous expression of cdgB in Escherichia coli resulted in increased production of extracellular matrix in colonies and enhanced surface attachment of cells in standing liquid cultures. In Streptomyces coelicolor, both overexpression and deletion of cdgB inhibited aerial-mycelium formation, and overexpression also inhibited production of the antibiotic actinorhodin, implicating c-di-GMP in the regulation of developmental processes in Streptomyces. |
| Links |
PubMed PMC3133206 Online version:10.1128/JB.01460-10 |
| Keywords |
Anthraquinones/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial/physiology; Gene Expression Regulation, Enzymologic/physiology; Phosphorus-Oxygen Lyases/genetics; Phosphorus-Oxygen Lyases/metabolism; Streptomyces coelicolor/enzymology; Streptomyces coelicolor/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0044010: single-species biofilm formation |
ECO:0000315: |
P |
Figure 6B-effect of cdgB induction on changes in biofilm formation by evaluating the intensity of crystal violet staining in E. Coli cells adhering to the glass surface at the air-liquid interface in static cultures. |
complete | ||||
|
involved_in |
GO:0044010: single-species biofilm formation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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