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PMID:21513512

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Citation

Wanarska, M, Krawczyk, B, Hildebrandt, P and Kur, J (2011) RecA proteins from Deinococcus geothermalis and Deinococcus murrayi--cloning, purification and biochemical characterisation. BMC Mol. Biol. 12:17

Abstract

Escherichia coli RecA plays a crucial role in recombinational processes, the induction of SOS responses and mutagenic lesion bypasses. It has also been demonstrated that RecA protein is indispensable when it comes to the reassembly of shattered chromosomes in γ-irradiated Deinococcus radiodurans, one of the most radiation-resistant organisms known. Moreover, some functional differences between E. coli and D. radiodurans RecA proteins have also been shown.

Links

PubMed PMC3103430 Online version:10.1186/1471-2199-12-17

Keywords

Adenosine Triphosphate/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Cloning, Molecular; DNA, Single-Stranded/metabolism; Deinococcus/genetics; Deinococcus/metabolism; Escherichia coli/genetics; Gene Expression; Genes, Bacterial; Hot Temperature; Molecular Sequence Data; Protein Binding; Protein Stability; Rec A Recombinases/genetics; Rec A Recombinases/isolation & purification; Rec A Recombinases/metabolism; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DEIGD:RECA

enables

GO:0043142: single-stranded DNA-dependent ATPase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DEIGD:RECA

GO:0043142: single-stranded DNA-dependent ATPase activity

ECO:0000314:

F

Table 3 shows the effect of DNA on the rate of ATP and dATP hydrolysis catalyzed by DgeRecA.

complete
CACAO 7148

9DEIO:E7CG23

enables

GO:0043142: single-stranded DNA-dependent ATPase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

9DEIO:E7CG23

GO:0043142: single-stranded DNA-dependent ATPase activity

ECO:0000314:

F

Table 3 shows the effect of DNA on the rate of ATP and dATP hydrolysis catalyzed by DgeRecA.

complete
CACAO 7149


See also

References

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