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PMID:21513512
Citation |
Wanarska, M, Krawczyk, B, Hildebrandt, P and Kur, J (2011) RecA proteins from Deinococcus geothermalis and Deinococcus murrayi--cloning, purification and biochemical characterisation. BMC Mol. Biol. 12:17 |
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Abstract |
Escherichia coli RecA plays a crucial role in recombinational processes, the induction of SOS responses and mutagenic lesion bypasses. It has also been demonstrated that RecA protein is indispensable when it comes to the reassembly of shattered chromosomes in γ-irradiated Deinococcus radiodurans, one of the most radiation-resistant organisms known. Moreover, some functional differences between E. coli and D. radiodurans RecA proteins have also been shown. |
Links |
PubMed PMC3103430 Online version:10.1186/1471-2199-12-17 |
Keywords |
Adenosine Triphosphate/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Cloning, Molecular; DNA, Single-Stranded/metabolism; Deinococcus/genetics; Deinococcus/metabolism; Escherichia coli/genetics; Gene Expression; Genes, Bacterial; Hot Temperature; Molecular Sequence Data; Protein Binding; Protein Stability; Rec A Recombinases/genetics; Rec A Recombinases/isolation & purification; Rec A Recombinases/metabolism; Sequence Alignment |
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Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
enables |
GO:0043142: single-stranded DNA-dependent ATPase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
GO:0043142: single-stranded DNA-dependent ATPase activity |
ECO:0000314: |
F |
Table 3 shows the effect of DNA on the rate of ATP and dATP hydrolysis catalyzed by DgeRecA. |
complete | ||||
enables |
GO:0043142: single-stranded DNA-dependent ATPase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
GO:0043142: single-stranded DNA-dependent ATPase activity |
ECO:0000314: |
F |
Table 3 shows the effect of DNA on the rate of ATP and dATP hydrolysis catalyzed by DgeRecA. |
complete | ||||
See also
References
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