PMID:21511764

Citation	Das, AD and Misra, HS (2011) Characterization of DRA0282 from Deinococcus radiodurans for its role in bacterial resistance to DNA damage. Microbiology (Reading, Engl.) 157:2196-205
Abstract	DRA0282, a hypothetical protein, was found in a pool of nucleotide-binding proteins in Deinococcus radiodurans cells recovering from gamma radiation stress. This pool exhibited an unusual inhibition of nuclease activity by ATP. The N terminus of DRA0282 showed similarity to human Ku80 homologues, while the C terminus showed no similarities to known proteins. The recombinant protein required Mn(2+) for its interaction with DNA and protected dsDNA from exonuclease III degradation. The binding of the protein to supercoiled DNA with a K(d) of ~2.93 nM was nearly 20-fold stronger than its binding to ssDNA and nearly 67-fold stronger than its binding to linear dsDNA. Escherichia coli cells expressing DRA0282 showed a RecA-dependent enhancement of UV and gamma radiation tolerance. The ΔdrA0282 mutant of D. radiodurans showed a dose-dependent response to gamma radiation. At 14 kGy, the ΔdrA0282 mutant showed nearly 10-fold less survival, while at this dose both pprA : : catΔdrA0282 and pprA : : cat mutants were nearly 100-fold more sensitive than the wild-type. These results suggested that DRA0282 is a DNA-binding protein with a preference for superhelical DNA, and that it plays a role in bacterial resistance to DNA damage through a pathway in which PprA perhaps plays a dominant role in D. radiodurans.
Links	PubMed Online version:10.1099/mic.0.040436-0
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cations, Divalent/metabolism; Cloning, Molecular; Coenzymes/metabolism; DNA/metabolism; DNA Damage; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Deinococcus/genetics; Deinococcus/metabolism; Deinococcus/radiation effects; Escherichia coli/genetics; Escherichia coli/radiation effects; Gene Deletion; Gene Expression; Kinetics; Manganese/metabolism; Microbial Viability/radiation effects; Models, Molecular; Protein Binding; Protein Conformation; Recombinant Proteins/genetics; Recombinant Proteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DEIRA:Q9RXM8	involved_in	GO:0042262: DNA protection	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
DEIRA:Q9RXM8		GO:0042262: DNA protection	ECO:0000314:		P		Figure 5	complete CACAO 7140

References

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