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PMID:21493812
| Citation |
Tamasloukht, B, Wong Quai Lam, MS, Martinez, Y, Tozo, K, Barbier, O, Jourda, C, Jauneau, A, Borderies, G, Balzergue, S, Renou, JP, Huguet, S, Martinant, JP, Tatout, C, Lapierre, C, Barrière, Y, Goffner, D and Pichon, M (2011) Characterization of a cinnamoyl-CoA reductase 1 (CCR1) mutant in maize: effects on lignification, fibre development, and global gene expression. J. Exp. Bot. 62:3837-48 |
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| Abstract |
Cinnamoyl-CoA reductase (CCR), which catalyses the first committed step of the lignin-specific branch of monolignol biosynthesis, has been extensively characterized in dicot species, but few data are available in monocots. By screening a Mu insertional mutant collection in maize, a mutant in the CCR1 gene was isolated named Zmccr1(-). In this mutant, CCR1 gene expression is reduced to 31% of the residual wild-type level. Zmccr1(-) exhibited enhanced digestibility without compromising plant growth and development. Lignin analysis revealed a slight decrease in lignin content and significant changes in lignin structure. p-Hydroxyphenyl units were strongly decreased and the syringyl/guaiacyl ratio was slightly increased. At the cellular level, alterations in lignin deposition were mainly observed in the walls of the sclerenchymatic fibre cells surrounding the vascular bundles. These cell walls showed little to no staining with phloroglucinol. These histochemical changes were accompanied by an increase in sclerenchyma surface area and an alteration in cell shape. In keeping with this cell type-specific phenotype, transcriptomics performed at an early stage of plant development revealed the down-regulation of genes specifically associated with fibre wall formation. To the present authors' knowledge, this is the first functional characterization of CCR1 in a grass species. |
| Links |
PubMed PMC3134344 Online version:10.1093/jxb/err077 |
| Keywords |
Aldehyde Oxidoreductases/genetics; Aldehyde Oxidoreductases/metabolism; Cell Wall/chemistry; Cell Wall/genetics; Cell Wall/metabolism; Gene Expression; Gene Expression Regulation, Plant; Immunohistochemistry; Lignin/biosynthesis; Lignin/chemistry; Lignin/genetics; Lignin/metabolism; Phylogeny; Plant Proteins/metabolism; Plants, Genetically Modified/genetics; Plants, Genetically Modified/growth & development; Plants, Genetically Modified/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Zea mays/genetics; Zea mays/growth & development; Zea mays/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0009809: lignin biosynthetic process |
ECO:0000315: |
P |
Table 1 and figure 2 show that in a mutant maize strain that has 31% of the wild type CCR expression, Klason Lignin extracted is significantly lower, lignin monomers are made in different ratios, and stained lignin is less abundant. |
complete | ||||
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involved_in |
GO:0009809: lignin biosynthetic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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