PMID:21464593

Citation	Guinote, IB, Matos, RG, Freire, P and Arraiano, CM (2011) BolA affects cell growth, and binds to the promoters of penicillin-binding proteins 5 and 6 and regulates their expression. J. Microbiol. Biotechnol. 21:243-51
Abstract	The gene bolA was discovered in the 80's, but unraveling its function in the cell has proven to be a complex task. The BolA protein has pleiotropic effects over cell physiology, altering growth and morphology, inducing biofilm formation, and regulating the balance of several membrane proteins. Recently, BolA was shown to be a transcription factor by repressing the expression of the mreB gene. The present report shows that BolA is a transcriptional regulator of the dacA and dacC genes, thus regulating both DD-carboxypeptidases PBP5 and PBP6 and thereby demonstrating the versatility of BolA as a cellular regulator. In this work, we also demonstrate that reduction of cell growth and survival can be connected to the overexpression of the bolA gene in different E. coli backgrounds, particularly in the exponential growth phase. The most interesting finding is that overproduction of BolA affects bacterial growth differently depending on whether the cells were inoculated directly from a plate culture or from an overnight batch culture. This strengthens the idea that BolA can be engaged in the coordination of genes that adapt the cell physiology in order to enhance cell adaptation and survival under stress conditions.
Links	PubMed
Keywords	Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Penicillin-Binding Proteins/biosynthesis; Promoter Regions, Genetic; Protein Binding; Transcription Factors/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status

References

See Help:References for how to manage references in GONUTS.