PMID:21457366

Citation	Vain, P, Thole, V, Worland, B, Opanowicz, M, Bush, MS and Doonan, JH (2011) A T-DNA mutation in the RNA helicase eIF4A confers a dose-dependent dwarfing phenotype in Brachypodium distachyon. Plant J. 66:929-40
Abstract	In a survey of the BrachyTAG mutant population of Brachypodium distachyon, we identified a line carrying a T-DNA insertion in one of the two eukaryotic initiation factor 4A (eIF4A) genes present in the nuclear genome. The eif4a homozygous mutant plants were slow-growing, and exhibited reduced final plant stature due to a decrease in both cell number and cell size, consistent with roles for eIF4A in both cell division and cell growth. Hemizygous plants displayed a semi-dwarfing phenotype, in which stem length was reduced but leaf length was normal. Linkage between the insertion site and phenotype was confirmed, and we show that the level of eIF4A protein is strongly reduced in the mutant. Transformation of the Brachypodium homozygous mutant with a genomic copy of the Arabidopsis eIF4A-1 gene partially complemented the growth phenotype, indicating that gene function is conserved between mono- and dicotyledonous species. This study identifies eIF4A as a novel dose-dependent regulator of stem elongation, and demonstrates the utility of Brachypodium as a model for grass and cereals research.
Links	PubMed Online version:10.1111/j.1365-313X.2011.04555.x
Keywords	Arabidopsis/genetics; Brachypodium/genetics; Brachypodium/growth & development; Brachypodium/ultrastructure; Cells, Cultured; Cloning, Molecular; DNA, Bacterial/genetics; DNA, Plant/metabolism; Eukaryotic Initiation Factor-4A/genetics; Eukaryotic Initiation Factor-4A/metabolism; Genes, Plant; Genetic Complementation Test; Genetic Vectors; Genotype; Inflorescence/growth & development; Mutagenesis, Insertional; Phenotype; Plant Leaves/ultrastructure; Plant Proteins/genetics; Plant Proteins/metabolism; Seeds/genetics; Seeds/growth & development
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BRADI:I1GWP1	involved_in	GO:0009826: unidimensional cell growth	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
BRADI:I1GWP1		GO:0009826: unidimensional cell growth	ECO:0000315:		P		Figure 6	complete CACAO 8002

References

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