PMID:21371996

Citation	Grainge, I, Lesterlin, C and Sherratt, DJ (2011) Activation of XerCD-dif recombination by the FtsK DNA translocase. Nucleic Acids Res. 39:5140-8
Abstract	The FtsK translocase pumps dsDNA directionally at ∼5 kb/s and facilitates chromosome unlinking by activating XerCD site-specific recombination at dif, located in the replication terminus of the Escherichia coli chromosome. We show directly that the γ regulatory subdomain of FtsK activates XerD catalytic activity to generate Holliday junction intermediates that can then be resolved by XerC. Furthermore, we demonstrate that γ can activate XerCD-dif recombination in the absence of the translocase domain, when it is fused to XerCD, or added in isolation. In these cases the recombination products are topologically complex and would impair chromosome unlinking. We propose that FtsK translocation and activation of unlinking are normally coupled, with the translocation being essential for ensuring that the products of recombination are topologically unlinked, an essential feature of the role of FtsK in chromosome segregation.
Links	PubMed PMC3130261 Online version:10.1093/nar/gkr078
Keywords	Chromosomes, Bacterial/metabolism; DNA/metabolism; DNA, Cruciform; Enzyme Activation; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Integrases/metabolism; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/metabolism; Protein Structure, Tertiary; Recombination, Genetic; Sequence Deletion
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:FTSK	involved_in	GO:0043085: positive regulation of catalytic activity	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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