PMID:21288493

Citation	Wang, X, Wang, Q, Yang, M, Xiao, J, Liu, Q, Wu, H and Zhang, Y (2011) QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda. Fish Shellfish Immunol. 30:944-53
Abstract	The inter-kingdom communication with the mammalian hosts mediated by autoinducer-3 (AI-3)/epinephrine (Epi)/norepinephrine (NE), and transduced by two-component systems QseBC has recently been described. As a fish pathogen and opportunistic pathogen for human beings, Edwardsiella tarda develops surface structures such as flagellar and fimbriae to cause different hemagglutination phenotypes and serotypes and initiate pathogen-host recognition and invasion process. E. tarda survives within macrophages in fish using type III secretion system (TTSS). Here, the genes of E. tarda two-component system, qseB and qseC, were found to be co-transcribed. Phylogenetic analysis indicated that evolution of QseC strongly correlated to different host niches. Compared with the wild type and their complemented strains, ΔqseB and ΔqseC mutants exhibited significant impaired flagellar motilities. Mammalian Epi was able to stimuli the flagellar motility in E. tarda via QseBC. Hemagglutination caused by fimbriae was induced in ΔqseB but repressed in ΔqseC. Disruption of qseB or qseC down-regulated the intracellular expressions of TTSS elements EseB and EsaC, and impaired their intracellular survival capabilities as well as in vivo competitive abilities. Furthermore, in vitro tests indicated that expression of EseB was induced by Epi via QseBC. Our results revealed that the QseBC system modified the virulence-related surface structures (flagellum, fimbriae and secretion system) and that hormone might stimulate the virulence of the pathogen in fish.
Links	PubMed Online version:10.1016/j.fsi.2011.01.019
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Secretion Systems/genetics; Edwardsiella tarda/genetics; Edwardsiella tarda/metabolism; Edwardsiella tarda/pathogenicity; Edwardsiella tarda/physiology; Fimbriae, Bacterial/genetics; Fimbriae, Bacterial/metabolism; Flagella/genetics; Flagella/physiology; Gene Order; Hemagglutination/genetics; Macrophages/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
EDWTA:E3UKS8		GO:2000154: negative regulation of flagellar cell motility	ECO:0000315:		P		Figure 2 shows a motility assay for the WT and QseBC deletion mutants.	complete CACAO 4943
EDWTA:E3UKS8		GO:0009405: pathogenesis	ECO:0000315:		P		Figure 4 shows a survival curve for WT and deletion mutants.	complete CACAO 4944
EDWTA:E3UKS8	involved_in	GO:0009405: pathogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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