PMID:21264466

Citation	Lai, MJ, Lin, NT, Hu, A, Soo, PC, Chen, LK, Chen, LH and Chang, KC (2011) Antibacterial activity of Acinetobacter baumannii phage ϕAB2 endolysin (LysAB2) against both gram-positive and gram-negative bacteria. Appl. Microbiol. Biotechnol. 90:529-39
Abstract	To investigate the nature and origin of the antibacterial activity of the lytic phage ϕAB2 toward Acinetobacter baumannii, we successfully isolated and characterized a novel phage lysozyme (endolysin) from ϕAB2 and named it LysAB2. To analyze antibacterial activity of LysAB2, the complete LysAB2 and two deletion derivatives were constructed, purified and characterized. Zymographic assays showed that only the intact LysAB2 could lyse the peptidoglycan of A. baumannii and the Staphylococcus aureus cell wall. Antibacterial analysis also showed that only the intact LysAB2 retained the complete bactericidal activity. When applied exogenously, LysAB2 exhibited a broad bacteriolytic activity against a number of Gram-negative and Gram-positive bacteria. Thermostability assays indicated that LysAB2 was stable at 20∼40 °C. Its optimal pH was 6.0, and it was active from pH 4 to 8. Scanning electron microscopy revealed that exposure to 500 μgml(-1) LysAB2 for up to 60 min caused a remarkable modification of the cell shape of the bacteria. Treating bacteria with LysAB2 clearly enhanced permeation of the bacterial cytoplasmic membrane. These results indicate that LysAB2 is an effective lysozyme against bacteria, and they suggest that it is a good candidate for a therapeutic/disinfectant agent to control nosocomial infections caused by multiple drug-resistant bacteria.
Links	PubMed Online version:10.1007/s00253-011-3104-y
Keywords	Acinetobacter baumannii/drug effects; Acinetobacter baumannii/growth & development; Acinetobacter baumannii/virology; Amino Acid Sequence; Anti-Bacterial Agents/pharmacology; Bacteriophages/enzymology; Cell Wall/drug effects; Drug Resistance, Multiple, Bacterial; Endopeptidases/pharmacology; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Gram-Positive Bacteria/drug effects; Gram-Positive Bacteria/growth & development; Molecular Sequence Data; Peptidoglycan/metabolism; Plasmids/genetics; Staphylococcus aureus/drug effects; Staphylococcus aureus/growth & development
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9VIRU:F1BCP4	involved_in	GO:0044659: cytolysis by virus of host cell	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
9VIRU:F1BCP4		GO:0044659: cytolysis by virus of host cell	ECO:0000314:		P		A. baumannii cells incubated with purified LysAB2 showed greater rates of cell lysis compared to the phosphate buffer control in the lysis curve (Figure 4).	complete CACAO 10908

References

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