PMID:21241518

Citation	Zheng, J, Wei, C, Zhao, L, Liu, L, Leng, W, Li, W and Jin, Q (2011) Combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem mass spectrometry as an effective strategy for analyzing potential membrane protein complexes of Mycobacterium bovis bacillus Calmette-Guérin. BMC Genomics 12:40
Abstract	Tuberculosis is an infectious bacterial disease in humans caused primarily by Mycobacterium tuberculosis, and infects one-third of the world's total population. Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccine has been widely used to prevent tuberculosis worldwide since 1921. Membrane proteins play important roles in various cellular processes, and the protein-protein interactions involved in these processes may provide further information about molecular organization and cellular pathways. However, membrane proteins are notoriously under-represented by traditional two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and little is known about mycobacterial membrane and membrane-associated protein complexes. Here we investigated M. bovis BCG by an alternative proteomic strategy coupling blue native PAGE to liquid chromatography tandem mass spectrometry (LC-MS/MS) to characterize potential protein-protein interactions in membrane fractions.
Links	PubMed PMC3032701 Online version:10.1186/1471-2164-12-40
Keywords	Bacterial Proteins/metabolism; Chromatography, Liquid/methods; Electrophoresis, Polyacrylamide Gel/methods; Membrane Proteins/metabolism; Mycobacterium bovis/metabolism; Pentosyltransferases/metabolism; Tandem Mass Spectrometry/methods
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MYCBP:ATPG	part_of	GO:0005886: plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MYCBP:ATPG		GO:0005886: plasma membrane	ECO:0000314:		C		Figure 3.	complete CACAO 7076

References

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