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PMID:21216904
| Citation |
Harreither, W, Sygmund, C, Augustin, M, Narciso, M, Rabinovich, ML, Gorton, L, Haltrich, D and Ludwig, R (2011) Catalytic properties and classification of cellobiose dehydrogenases from ascomycetes. Appl. Environ. Microbiol. 77:1804-15 |
|---|---|
| Abstract |
Putative cellobiose dehydrogenase (CDH) genes are frequently discovered in various fungi by genome sequencing projects. The expression of CDH, an extracellular flavocytochrome, is well studied in white rot basidiomycetes and is attributed to extracellular lignocellulose degradation. CDH has also been reported for plant-pathogenic or saprotrophic ascomycetes, but the molecular and catalytic properties of these enzymes are currently less investigated. This study links various ascomycetous cdh genes with the molecular and catalytic characteristics of the mature proteins and suggests a differentiation of ascomycete class II CDHs into two subclasses, namely, class IIA and class IIB, in addition to the recently introduced class III of hypothetical ascomycete CDHs. This new classification is based on sequence and biochemical data obtained from sequenced fungal genomes and a screening of 40 ascomycetes. Thirteen strains showed CDH activity when they were grown on cellulose-based media, and Chaetomium atrobrunneum, Corynascus thermophilus, Dichomera saubinetii, Hypoxylon haematostroma, Neurospora crassa, and Stachybotrys bisbyi were selected for detailed studies. In these strains, one or two cdh-encoding genes were found that stem either from class IIA and contain a C-terminal carbohydrate-binding module or from class IIB without such a module. In several strains, both genes were found. Regarding substrate specificity, class IIB CDHs show a less pronounced substrate specificity for cellobiose than class IIA enzymes. A pH-dependent pattern of the intramolecular electron transfer was also observed, and the CDHs were classified into three groups featuring acidic, intermediate, or alkaline pH optima. The pH optimum, however, does not correlate with the CDH subclasses and is most likely a species-dependent adaptation to different habitats. |
| Links |
PubMed PMC3067291 Online version:10.1128/AEM.02052-10 |
| Keywords |
Amino Acid Sequence; Ascomycota/enzymology; Base Sequence; Carbohydrate Dehydrogenases/classification; Carbohydrate Dehydrogenases/genetics; Carbohydrate Dehydrogenases/isolation & purification; Carbohydrate Dehydrogenases/metabolism; Cellulose/metabolism; Culture Media/chemistry; DNA, Fungal; Kinetics; Molecular Sequence Data; Sequence Analysis, DNA; Substrate Specificity |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: |
F |
Table 3 shows kinetic constants of the CDH activity of CDH IIA. (The accession number of NcCDH IIA is given in table 2) |
complete | ||||
|
enables |
GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: |
F |
Table 3 shows kinetic constants of CDH activity of the purified CaCDH IIA. (accession number of CaCDH IIA is in table 2). |
complete | ||||
|
enables |
GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: |
F |
Table 3 shows kinetic constants of the CDH activity of purified DsCDH IIA (the accession number of which is in table 2). |
complete | ||||
|
enables |
GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: |
F |
Table 3 shows kinetic constants of CDH activity of purified HhCDH IIB (its accession number is found in table 2). |
complete | ||||
|
enables |
GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: |
F |
Table 3 shows the kinetic constants of CDH activity of CtCDH IIB. (the accession number of CtCDH IIB is in table 2). |
complete | ||||
|
enables |
GO:0047735: cellobiose dehydrogenase (acceptor) activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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